Lü Peng, Xing Yali, Hu Zhaoyang, Yang Yanhua, Pan Ye, Chen Kangmin, Zhu Feifei, Zhou Yajing, Chen Keping, Yao Qin
Institute of Life Sciences, Jiangsu University, Zhenjiang 212013, China.
Institute of Life Sciences, Jiangsu University, Zhenjiang 212013, China; Laboratory Animal Research Center, Jiangsu University, Zhenjiang 212013, China.
J Invertebr Pathol. 2017 Mar;144:18-23. doi: 10.1016/j.jip.2016.12.008. Epub 2017 Jan 2.
Bombyx mori bidensiovirus (BmBDV) is a species of Bidensovirus that has been was placed into a new genus within the new family Bidnaviridae by the International Committee on Taxonomy of Viruses. BmBDV causes fatal flacherie disease in silkworms, which causes large losses to the sericulture industry. BmBDV contains two sets of complementary linear single-stranded DNAs of approximately 6.5kb (viral DNA 1, VD1) and 6.0kb (viral DNA 2, VD2). VD1 and VD2 are encapsidated in separate icosahedral non-enveloped capsids, which are similar in size and shape. However, the strategies used to express BmBDV structural proteins remains unclear. In this work, a total of six structural proteins were separated by two-dimensional electrophoresis and shown to be encoded by the BmBDV VP gene via mass spectrometry. The transmission electron microscopy results showed that co-expression of the BmBDV VP and SP structural proteins in Spodoptera frugiperda sf9 cells resulted in the formation of 22-24nm virus-like particles. Furthermore, a mutation of the major structural protein-encoding VP gene, in which the second in-frame ATG codon was mutated to GCG, abrogated the production of several structural proteins, indicating that this strategy of expressing BmBDV VP is dependent on a leaky scanning translation mechanism.
家蚕双顺反子病毒（BmBDV）是双顺反子病毒属的一个物种，已被国际病毒分类委员会归入新科双顺反子病毒科的一个新属。BmBDV在家蚕中引起致命的空头性软化病，给养蚕业造成巨大损失。BmBDV包含两组互补的线性单链DNA，大小约为6.5kb（病毒DNA 1，VD1）和6.0kb（病毒DNA 2，VD2）。VD1和VD2被包裹在单独的二十面体无包膜衣壳中，衣壳大小和形状相似。然而，BmBDV结构蛋白的表达策略仍不清楚。在这项工作中，通过二维电泳分离出总共六种结构蛋白，并通过质谱分析表明它们由BmBDV VP基因编码。透射电子显微镜结果显示，BmBDV VP和SP结构蛋白在草地贪夜蛾sf9细胞中共表达导致形成22 - 24nm的病毒样颗粒。此外，主要结构蛋白编码VP基因的一个突变，其中第二个读码框内的ATG密码子突变为GCG，消除了几种结构蛋白的产生，表明这种表达BmBDV VP的策略依赖于渗漏扫描翻译机制。
