Institute of Life Sciences, Jiangsu University, Zhenjiang 212013, China.
Viruses. 2019 Apr 3;11(4):325. doi: 10.3390/v11040325.
bidensovirus (BmBDV) is a single-stranded DNA (ssDNA) virus from the genus of the Bidnaviridae family, which, thus far, solely infects insects. It has a unique genome that contains bipartite DNA molecules (VD1 and VD2). In this study, we explored the detailed transcription mapping of the complete BmBDV genome (VD1 and VD2) by rapid amplification of cDNA ends (RACE), reverse transcription quantitative real-time PCR (RT-qPCR), and luciferase assays. For the first time, we report the transcription map of VD2. Our mapping of the transcriptional start sites reveals that the NS genes in VD1 have separate transcripts that are derived from overlapping promoters, P5 and P5.5. Thus, our study provides a strategy for alternative promoter usage in the expression of BmBDV genes.
双瓣病毒（BmBDV）是杆状病毒科双瓣病毒属的一种单链 DNA（ssDNA）病毒，迄今为止，它仅感染昆虫。它具有独特的基因组，包含二分体 DNA 分子（VD1 和 VD2）。在这项研究中，我们通过快速扩增 cDNA 末端（RACE）、反转录定量实时 PCR（RT-qPCR）和荧光素酶测定法，探索了完整 BmBDV 基因组（VD1 和 VD2）的详细转录图谱。我们首次报道了 VD2 的转录图谱。我们对转录起始位点的定位揭示了 VD1 中的 NS 基因具有来自重叠启动子 P5 和 P5.5 的单独转录物。因此，我们的研究为 BmBDV 基因表达中替代启动子的使用提供了一种策略。
