Di Ciccio Pierluigi, Ossiprandi Maria Cristina, Zanardi Emanuela, Ghidini Sergio, Belluzzi Giancarlo, Vergara Alberto, Ianieri Adriana
Department of Food Science, University of Parma , Parma.
Department of Veterinary Science, University of Parma.
Ital J Food Saf. 2016 Nov 2;5(4):6151. doi: 10.4081/ijfs.2016.6151. eCollection 2016 Sep 20.
The aim of this survey was to obtain data on microbiological contamination of pig carcasses and environments in three large-scale Italian slaughterhouses (identified as A-B-C) located in Northern Italy. Each slaughterhouse was visited six times. Five carcasses and three different sites of the slaughterhouse (before and during slaughter) were sampled on each sampling day. A single pooled caecal sample was taken on each sampling day. A total of 90 carcasses, 108 environmental samples and 18 caecal samples were collected. Samples from pig carcasses and slaughterhouse environment were analyzed for total viable count (TVC), count (EBC) and The caecal contents were examined for Carcasses from slaughterhouse A presented the greatest TVC and EBC mean log value, whereas environmental samples collected during slaughter activities from slaughterhouse C showed the greatest TVC and EBC mean log value. As far as the environmental samples collected before slaughter activities are concerned, an average up to 6 log colony forming unit (CFU)/cm TVC in two slaughter plants (A and C) and 5 log CFU/cm TVC in one slaughter plant (B) was detected. was recovered in two slaughterhouses (A and B). Four different serotypes were detected in the positive samples (11). Within serotype Rissen and Typhimurium monophasic-variant isolates, two pulsed-field gel electrophoresis patterns were identified. The findings in this survey suggest that carcass contamination is influenced by the slaughterhouse plant and this could be a result of differences in line speed. The results of environmental sampling have not shown an association with the slaughterhouse plant.
本次调查的目的是获取位于意大利北部的三家大型意大利屠宰场（分别标识为A - B - C）生猪胴体和环境的微生物污染数据。每家屠宰场均被走访六次。在每个采样日，对五具胴体以及屠宰场的三个不同地点（屠宰前和屠宰期间）进行采样。每个采样日采集一份盲肠混合样本。总共收集了90具胴体样本、108份环境样本和18份盲肠样本。对生猪胴体和屠宰场环境样本进行了总活菌数（TVC）、大肠杆菌计数（EBC）分析，对盲肠内容物进行了[此处原文缺失具体检测项目]检测。屠宰场A的胴体呈现出最高的TVC和EBC平均对数值，而屠宰场C在屠宰活动期间采集的环境样本显示出最高的TVC和EBC平均对数值。就屠宰活动前采集的环境样本而言，在两家屠宰场（A和C）检测到平均高达6 log菌落形成单位（CFU）/平方厘米的TVC，在一家屠宰场（B）检测到5 log CFU/平方厘米的TVC。[此处原文缺失具体检测项目]在两家屠宰场（A和B）中被检出。在阳性样本中检测到四种不同的[此处原文缺失具体菌名]血清型（11）。在血清型[此处原文缺失具体血清型名称]中，鉴定出里森氏菌和单相变异鼠伤寒沙门氏菌的两种脉冲场凝胶电泳图谱。本次调查结果表明，胴体污染受屠宰场影响，这可能是生产线速度差异导致的。环境采样结果未显示与屠宰场有关联。
