弱精子症患者精子和精浆中丙二醛水平及其与精液参数的关系。
Malondialdehyde levels in sperm and seminal plasma of asthenozoospermic and its relationship with semen parameters.
作者信息
Tavilani Heidar, Doosti Mahmoud, Saeidi Hojatollah
机构信息
Department of Medical Biochemistry, Faculty of Medicine, Tehran University of Medical Science, Tehran, Iran.
出版信息
Clin Chim Acta. 2005 Jun;356(1-2):199-203. doi: 10.1016/j.cccn.2005.01.017. Epub 2005 Apr 9.
BACKGROUND
Toxic lipid peroxides are known to cause various impairments of sperm cells and may play a major role in the etiology of male infertility. We determined the level of lipid peroxidation as indicated by malondialdehyde (MDA) in the spermatozoa and seminal plasma of asthenozoospermic and normozoospermic males.
METHODS
MDA of spermatozoa and seminal plasma was determined in 35 asthenozoospermic and 15 normozoospermic men by spectrofluorometry. Semen analysis was done according to the WHO standard.
RESULTS
MDA concentration in the spermatozoa of asthenozoospermic was significantly higher than in normozoospermic males (0.14+/-0.059 and 0.09+/-0.04 nmol/10 x 10(6) spermatozoa respectively). The mean+/-S.D. MDA value in the seminal plasma of asthenozoospermic and normozoospermic were 1.35+/-0.42 and 1.2+/-0.3 nmol/ml seminal plasma respectively.
CONCLUSIONS
Lipid peroxidation has a deleterious effect on the semen quality and MDA is an index of lipid peroxidation which may be a diagnostic tool for the analysis of infertility in the asthenozoospermic patients.
背景
已知有毒的脂质过氧化物会导致精子细胞出现各种损伤，并且可能在男性不育症的病因中起主要作用。我们测定了弱精子症男性和正常精子症男性精子及精浆中丙二醛（MDA）所指示的脂质过氧化水平。
方法
采用荧光分光光度法测定了35例弱精子症男性和15例正常精子症男性精子及精浆中的MDA。精液分析按照世界卫生组织标准进行。
结果
弱精子症男性精子中的MDA浓度显著高于正常精子症男性（分别为0.14±0.059和0.09±0.04 nmol/10×10⁶精子）。弱精子症和正常精子症男性精浆中的平均±标准差MDA值分别为1.35±0.42和1.2±0.3 nmol/ml精浆。
结论
脂质过氧化对精液质量有有害影响，MDA是脂质过氧化的一个指标，可能是分析弱精子症患者不育症的一种诊断工具。
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