Agronomy Department, University of Florida, Gainesville, FL 32610, USA.
State Key Laboratory of Plant Physiology and Biochemistry, National Center for Evaluation of Agricultural Wild Plants (Rice), China Agricultural University, Beijing 100193, China.
Sci Rep. 2017 Jan 6;7:40066. doi: 10.1038/srep40066.
The molecular mechanisms of symbiosis in cultivated peanut with a 'crack entry' infection process are largely understudied. In this study, we investigated the root transcriptional profiles of two pairs of non-nodulating (nod-) and nodulating (nod+) sister inbred peanut lines, E4/E5 and E7/E6, and their nod+ parents, F487A and PI262090 during rhizobial infection and nodule initiation by using RNA-seq technology. A total of 143, 101, 123, 215, 182, and 289 differentially expressed genes (DEGs) were identified in nod- E4, E7 and nod+ E5, E6, F487A, and PI262090 after inoculation with Bradyrhizobium sp. Different deficiencies at upstream of symbiotic signaling pathway were revealed in the two nod- genotypes. DEGs specific in nod+ genotypes included orthologs to some known symbiotic signaling pathway genes, such as NFR5, NSP2, NIN, ERN1, and many other novel and/or functionally unknown genes. Gene ontology (GO) enrichment analysis of nod+ specific DEGs revealed 54 significantly enriched GO terms, including oxidation-reduction process, metabolic process, and catalytic activity. Genes related with plant defense systems, hormone biosynthesis and response were particularly enriched. To our knowledge, this is the first report revealing symbiosis-related genes in a genome-wide manner in peanut representative of the 'crack entry' species.
栽培花生与“裂缝进入”感染过程共生的分子机制在很大程度上仍未得到充分研究。在这项研究中，我们使用 RNA-seq 技术研究了两对非结瘤（nod-）和结瘤（nod+）姐妹自交系花生 E4/E5 和 E7/E6 及其 nod+亲本 F487A 和 PI262090 在根瘤菌感染和根瘤起始过程中的转录组。在接种慢生根瘤菌后，nod- E4、E7 和 nod+ E5、E6、F487A 和 PI262090 中分别鉴定出 143、101、123、215、182 和 289 个差异表达基因（DEGs）。在两个 nod-基因型中揭示了共生信号通路上游的不同缺陷。nod+基因型特有的 DEGs 包括一些已知共生信号通路基因的同源物，如 NFR5、NSP2、NIN、ERN1 和许多其他新的和/或功能未知的基因。对 nod+特异 DEGs 的基因本体（GO）富集分析显示，有 54 个显著富集的 GO 术语，包括氧化还原过程、代谢过程和催化活性。与植物防御系统、激素生物合成和响应相关的基因特别丰富。据我们所知，这是首次在代表“裂缝进入”物种的花生中以全基因组的方式揭示共生相关基因的报告。
