Department of Biochemistry, University of Calcutta, 35, Ballygunge Circular Road, Kolkata, 700019, India.
J Plant Res. 2021 Mar;134(2):307-326. doi: 10.1007/s10265-021-01256-w. Epub 2021 Feb 8.
Jasmonate ZIM domain (JAZ) proteins are the key negative regulators of jasmonate signaling, an important integrator of plant-microbe relationships. Versatility of jasmonate signaling outcomes are maintained through the multiplicity of JAZ proteins and their definitive functionalities. How jasmonate signaling influences the legume-Rhizobium symbiotic relationship is still unclear. In Arachis hypogaea (peanut), a legume plant, one JAZ sub-family (JAZ1) gene and one TIFY sequence containing protein family member (TIFY8) gene show enhanced expression in the early stage and late stage of root nodule symbiosis (RNS) respectively. In plants, JAZ sub-family proteins belong to a larger TIFY family. Here, this study denotes the first attempt to reveal in planta interactions of downstream jasmonate signaling regulators through proteomics and mass spectrometry to find out the mode of jasmonate signaling participation in the RNS process of A. hypogaea. From 4-day old Bradyrhizobium-infected peanut roots, the JAZ1-protein complex shows its contribution towards the rhizobial entry, nodule development, autoregulation of nodulation and photo-morphogenesis during the early stage of symbiosis. From 30-day old Bradyrhizobium infected roots, the TIFY8-protein complex reveals repressor functionality of TIFY8, suppression of root jasmonate signaling, modulation of root circadian rhythm and nodule development. Cellular localization and expression level of the interaction partners during the nodulation process further substantiate the in planta interaction pairs. This study provides a comprehensive insight into the jasmonate functionality in RNS through modulation of nodule number and development, during the early stage and root circadian rhythm during the late stage of nodulation, through the protein complexes of JAZ1 and TIFY8 respectively in A. hypogaea.
茉莉酸 ZIM 结构域（JAZ）蛋白是茉莉酸信号的关键负调控因子，是植物-微生物关系的重要整合因子。茉莉酸信号转导结果的多功能性是通过 JAZ 蛋白的多样性及其明确的功能来维持的。茉莉酸信号如何影响豆科植物-根瘤菌共生关系尚不清楚。在豆科植物花生中，一个 JAZ 亚家族（JAZ1）基因和一个含有 TIFY 序列的蛋白家族成员（TIFY8）基因分别在根瘤共生（RNS）的早期和晚期表现出增强的表达。在植物中，JAZ 亚家族蛋白属于更大的 TIFY 家族。本研究首次试图通过蛋白质组学和质谱法揭示下游茉莉酸信号转导调节剂的体内相互作用，以找出茉莉酸信号参与花生 RNS 过程的方式。从感染根瘤菌的 4 天大的花生根中，JAZ1-蛋白复合物显示其对根瘤菌进入、结瘤发育、结瘤的自身调节和共生早期的光形态发生的贡献。从感染根瘤菌的 30 天大的花生根中，TIFY8-蛋白复合物揭示了 TIFY8 的抑制子功能、根中茉莉酸信号的抑制、根昼夜节律的调制和结瘤发育。在结瘤过程中相互作用伙伴的细胞定位和表达水平进一步证实了体内相互作用对。本研究通过 JAZ1 和 TIFY8 蛋白复合物分别在花生中调节结瘤数和发育、早期的根昼夜节律，提供了对 RNS 中茉莉酸功能的全面了解。
