Jabir Nasimudeen R, Firoz Chelapram K, Kamal Mohammad A, Damanhouri Ghazi A, Alama Mohammed Nabil, Alam Qamre, Haque Absarul, Almehdar Hussein A, Tabrez Shams
King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia.
Faculty of Medicine, Department of Cardiology, King Abdulaziz University Hospital, Jeddah, Kingdom of Saudi Arabia.
J Cell Biochem. 2017 Jul;118(7):1849-1854. doi: 10.1002/jcb.25870. Epub 2017 Feb 16.
The purpose of the current study was to find out the possible changes polymorphic site at the promoter region of IL-18 gene in Saudi CAD patients. We have also measured serum IL-18 level to find out, the likely association between its level and polymorphic site. The present study included total 197 subjects (98 confirmed CAD patients both men and women and 99 healthy control individuals). Serum concentration of IL-18 was measured by enzyme linked immuno-sorbent assay. For SNPs analysis, sanger method of DNA sequencing was followed. We observed variable numbers of SNPs at -137 C/G, -607 A/C, and -656 T/G promoter sites in our studied samples. However, the observed changes in the number of SNP hotspots were found to be non-significant compared with control. IL-18 level was found to be significantly (P < 0.001) elevated in CAD patients compared with control individuals. The highest rise of around 36% (P < 0.001) in IL-18 level was recorded in unstable angina (UA) patients. Moreover, the group belonging to UA and non-ST segment elevation myocardial infarction (NSTEMI) showed only 6% rise. On the basis of our result, inflammation seems to have a role in the pathogenesis of CAD but not leading to the significant changes at the genetic level. J. Cell. Biochem. 118: 1849-1854, 2017. © 2017 Wiley Periodicals, Inc.
本研究的目的是找出沙特冠状动脉疾病（CAD）患者白细胞介素-18（IL-18）基因启动子区域多态性位点的可能变化。我们还测量了血清IL-18水平，以找出其水平与多态性位点之间可能存在的关联。本研究共纳入197名受试者（98名确诊的CAD患者，包括男性和女性，以及99名健康对照个体）。采用酶联免疫吸附测定法测量血清IL-18浓度。对于单核苷酸多态性（SNP）分析，采用桑格DNA测序法。在我们研究的样本中，我们在-137 C/G、-607 A/C和-656 T/G启动子位点观察到不同数量的SNP。然而，与对照组相比，观察到的SNP热点数量变化无统计学意义。与对照个体相比，CAD患者的IL-18水平显著升高（P < 0.001）。不稳定型心绞痛（UA）患者的IL-18水平升高幅度最大，约为36%（P < 0.001）。此外，UA和非ST段抬高型心肌梗死（NSTEMI）组的升高幅度仅为6%。根据我们的结果，炎症似乎在CAD的发病机制中起作用，但不会导致基因水平的显著变化。《细胞生物化学杂志》118: 1849 - 1854, 2017年。© 2017威利期刊公司。
