Deyhle Michael R, Sorensen Jacob R, Hyldahl Robert D
Exercise Sciences, Brigham Young University;
Exercise Sciences, Brigham Young University.
J Vis Exp. 2016 Dec 11(118):54859. doi: 10.3791/54859.
Contraction-induced muscle damage via voluntary eccentric (lengthening) contractions offers an excellent model for studying muscle adaptation and recovery in humans. Herein we discuss the design of an eccentric exercise protocol to induce damage in the quadriceps muscles, marked by changes in strength, soreness, and plasma creatine kinase levels. This method is simple, ethical, and widely applicable since it is performed in human participants and eliminates the interspecies translation of the results. Subjects perform 300 maximal eccentric contractions of the knee extensor muscles at a speed of 120°/sec on an isokinetic dynamometer. The extent of the damage is measurable using relatively non-invasive isokinetic and isometric measures of strength loss, soreness, and plasma creatine kinase levels over several days following the exercise. Therefore, its application can be directed to specific populations in an attempt to identify mechanisms for muscle adaptation and regeneration.
通过自愿性离心（拉长）收缩引起的肌肉损伤为研究人类肌肉适应和恢复提供了一个极佳的模型。在此，我们讨论一种离心运动方案的设计，该方案可诱导股四头肌损伤，其标志为力量、酸痛感和血浆肌酸激酶水平的变化。此方法简单、符合伦理且广泛适用，因为它是在人类受试者身上进行的，消除了种间结果转换的问题。受试者在等速测力计上以120°/秒的速度对膝伸肌进行300次最大离心收缩。损伤程度可通过在运动后数天内使用相对非侵入性的等速和等长力量损失测量、酸痛感测量以及血浆肌酸激酶水平测量来确定。因此，其应用可针对特定人群，以试图确定肌肉适应和再生的机制。
