Immunization of Mice.

The selection of an appropriate immunization strategy depends largely on the properties of an antigen, including its nature, purity, solubility, and availability. This unit describes the critical steps in the production of monoclonal and polyclonal antibodies against soluble molecules (such as proteins, peptides, polysaccharides, oligosaccharides, or hapten-conjugate vaccines), complex antigens (such as whole pathogens or outer membrane vesicles), and antigens embedded in or eluted from gel matrix following electrophoresis. © 2017 by John Wiley & Sons, Inc.