Kanda T, Iseki S, Hitomi M, Kimura H, Odani S, Kondo H, Matsubara Y, Muto T, Ono T
Department of Biochemistry, Niigata University School of Medicine, Japan.
Eur J Biochem. 1989 Oct 20;185(1):27-33. doi: 10.1111/j.1432-1033.1989.tb15076.x.
Fatty acid-binding protein (FABP) was purified from rat gastric mucosa by successive Sephadex G-75 chromatography, DEAE-cellulose chromatography and HPLC on an RP-2 (Merck) reversed-phase column. The purified stomach FABP migrated as a single band corresponding to an apparent molecular mass of 15 kDa on SDS/PAGE. Stomach FABP appeared to be identical with rat heart FABP, as judged from its electrophoretic mobility, amino acid composition and tryptic peptide map. In addition, the amino acid sequences of two selected tryptic peptides coincided completely with the rat heart FABP sequence deduced from that of cDNA. Stomach FABP showed immunochemical identity with rat heart FABP when tested with an antiserum against rat heart FABP. Immunohistochemically, stomach FABP was specifically stained with anti-(rat heart FABP) serum in parietal cells of the gastric mucosa. The results suggested that the primary structure of stomach FABP is identical with that of rat heart FABP, and showed that stomach FABP is localized in parietal cells of the gastric mucosa.
通过在葡聚糖G - 75柱上连续进行凝胶过滤层析、在DEAE - 纤维素柱上进行离子交换层析以及在RP - 2（默克公司）反相柱上进行高效液相色谱，从大鼠胃黏膜中纯化出脂肪酸结合蛋白（FABP）。纯化后的胃FABP在SDS / PAGE上迁移为一条单一的条带，其表观分子量为15 kDa。从其电泳迁移率、氨基酸组成和胰蛋白酶肽图谱判断，胃FABP似乎与大鼠心脏FABP相同。此外，两个选定的胰蛋白酶肽的氨基酸序列与从cDNA推导的大鼠心脏FABP序列完全一致。当用抗大鼠心脏FABP抗血清检测时，胃FABP与大鼠心脏FABP表现出免疫化学同一性。免疫组织化学研究表明，胃FABP在胃黏膜壁细胞中被抗（大鼠心脏FABP）血清特异性染色。结果表明，胃FABP的一级结构与大鼠心脏FABP相同，并表明胃FABP定位于胃黏膜的壁细胞中。
