Wang He, Wang Yunxiang, Yang Ruijin
Jiyang College, Zhejiang Agriculture and Forestry University, Zhuji, Zhejiang, 311800, China.
School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, 214122, China.
Appl Microbiol Biotechnol. 2017 Feb;101(3):933-949. doi: 10.1007/s00253-016-8080-9. Epub 2017 Jan 6.
With the increased knowledge on spore structure and advances in biotechnology engineering, the newly developed spore-surface display system confers several inherent advantages over other microbial cell-surface display systems including enhanced stability and high safety. Bacillus subtilis is the most commonly used Bacillus species for spore-surface display. The expression of heterologous antigen or protein on the surface of B. subtilis spores has now been practiced for over a decade with noteworthy success. As an update and supplement to other previous reviews, we comprehensively summarize recent studies in the B. subtilis spore-surface display technique. We focus on its benefits as well as the critical factors affecting its display efficiency and offer suggestions for the future success of this field.
随着对孢子结构的认识不断增加以及生物技术工程的进步，新开发的孢子表面展示系统相对于其他微生物细胞表面展示系统具有若干固有优势，包括更高的稳定性和高安全性。枯草芽孢杆菌是孢子表面展示最常用的芽孢杆菌种类。在枯草芽孢杆菌孢子表面表达异源抗原或蛋白质的实践已经进行了十多年，并取得了显著成功。作为对以往其他综述的更新和补充，我们全面总结了枯草芽孢杆菌孢子表面展示技术的最新研究。我们重点关注其优势以及影响其展示效率的关键因素，并为该领域未来的成功提供建议。
