Khan Muhammad Imran, Lee Moon Geon, Shin Jin Hyuk, Kim Jong Deog
Department of Biotechnology, Chonnam Natational University, San96-1, Dun-Duk Dong, Yeosu, Chonnam, 550-749, Korea.
Research Center on Anti-Obesity and Health Care, Chonnam National University, San96-1, Dun-Duk Dong, Yeosu, Chonnam, 550-749, Korea.
AMB Express. 2017 Dec;7(1):19. doi: 10.1186/s13568-016-0320-y. Epub 2017 Jan 7.
Microalgae are considered to be the future promising sources of biofuels and bio products. The algal carbohydrates can be fermented to bioethanol after pretreatment process. Efficient pretreatment of the biomass is one of the major requirements for commercialization of the algal based biofuels. In present study the microalga, M. aeruginsa was used for pretreatment optimization and bioethanol production. Treatment of algal biomass with CaO before acid and/or enzymatic hydrolysis enhanced the degradation of algal cells. Monomeric sugars yield was increased more than twice when biomass was pretreated with CaO. Similarly, an increase was noted in the amount of fermentable sugars when biomass was subjected to invertase saccharification after acid or lysozyme pretreatment. Highest yield of fermentable sugars (16 mM/ml) in the centrifuged algal juice was obtained. 4 Different microorganisms' species were used individually and in combination for converting centrifuged algal juice to bioethanol. Comparatively higher yield of bioethanol (60 mM/ml) was obtained when the fermenter microorganisms were used in combination. The results demonstrated that M. arginase biomass can be efficiently pretreated to get higher yield of fermentable sugars for enhanced yield of bioethanol production.
微藻被认为是未来有前景的生物燃料和生物产品来源。藻类碳水化合物经过预处理后可发酵生成生物乙醇。生物质的高效预处理是基于藻类的生物燃料商业化的主要要求之一。在本研究中，铜绿微囊藻用于预处理优化和生物乙醇生产。在酸和/或酶水解之前用CaO处理藻类生物质可增强藻类细胞的降解。当生物质用CaO预处理时，单糖产量增加了两倍多。同样，当生物质在酸或溶菌酶预处理后进行转化酶糖化时，可发酵糖的量也有所增加。在离心后的藻汁中获得了最高产量的可发酵糖（16 mM/ml）。分别使用4种不同的微生物种类，并将它们组合起来用于将离心后的藻汁转化为生物乙醇。当使用组合的发酵微生物时，获得了相对较高产量的生物乙醇（60 mM/ml）。结果表明，精氨酸酶生物质可以进行高效预处理，以获得更高产量的可发酵糖，从而提高生物乙醇的产量。
