Ujaoney Shweta, Chandra Jyotsna, Faddoul Fady, Chane Maya, Wang Jing, Taifour Louay, Mamtani Manju R, Thakre Tushar P, Kulkarni Hemant, Mukherjee Pranab, Ghannoum Mahmoud A
J Dent Hyg. 2014 Jun;88(3):183-9.
There is a burgeoning recognition and interest in the potential of probiotics in the treatment and prevention of oral candidiasis associated with the use of dentures. Our aim was to investigate if commercially available over-the-counter probiotics can influence the ability of Candida albicans to form biofilms, which is considered a hallmark of the initiation and progression of oral candidiasis.
We conducted a 2×5 factorial in vitro study to culture C. albicans on denture strips and challenge with one of the following four commercially available probiotics in bacterial or cell-free supernatant form: Accuflora®, Align®, Culturelle® and Sustenex®. C. albicans biofilm formation was studied in triplicates in all factorial combinations of the study and assessed qualitatively with fluorescence microscopy and quantitatively with tetrazolium salt (XTT) reduction assay. Quality control measures included determination of coefficient of variation, Bland Altman plots and Pittman's test. Results were analyzed using two-way analysis of variance (ANOVA) with pairwise post-hoc Scheffe's tests.
Our experimental conditions passed the quality control checks. Two-way ANOVA results indicated that cell-free supernatants provided a stronger and significant inhibitory effect on biofilm formation than their bacterial counterparts (2-way ANOVA p=3.8×10(-6)). Further, Lactobacillus-containing probiotic formulations (Accuflora® and Culturelle®) significantly reduced biofilm formation especially in supernatant form.
Commercially available probiotics that contain Lactobacilli species interfere with the in vitro ability of C. albicans to form biofilms on dentures. The mechanistic and clinical implications of our results need to be addressed by larger in vivo studies.
人们日益认识到益生菌在治疗和预防与假牙使用相关的口腔念珠菌病方面的潜力，并对此产生了兴趣。我们的目的是研究市售的非处方益生菌是否会影响白色念珠菌形成生物膜的能力，而生物膜形成被认为是口腔念珠菌病发生和发展的一个标志。
我们进行了一项2×5析因体外研究，将白色念珠菌接种在假牙条上，并用以下四种市售益生菌中的一种以细菌或无细胞上清液形式进行挑战：Accuflora®、Align®、Culturelle®和Sustenex®。在该研究的所有析因组合中，对白色念珠菌生物膜形成进行了三次重复研究，并通过荧光显微镜进行定性评估，通过四氮唑盐（XTT）还原试验进行定量评估。质量控制措施包括变异系数的测定、布兰德 - 奥特曼图和皮特曼检验。使用双向方差分析（ANOVA）和两两事后谢弗检验对结果进行分析。
我们的实验条件通过了质量控制检查。双向方差分析结果表明，无细胞上清液对生物膜形成的抑制作用比其细菌对应物更强且具有显著意义（双向方差分析p = 3.8×10⁻⁶）。此外，含乳酸杆菌的益生菌制剂（Accuflora®和Culturelle®）显著减少了生物膜形成，尤其是在上清液形式下。
市售的含乳酸杆菌的益生菌会干扰白色念珠菌在体外于假牙上形成生物膜的能力。我们结果的机制和临床意义需要通过更大规模的体内研究来探讨。
