Ji Pengchao, Liu Yunchao, Chen Yumei, Wang Aiping, Jiang Dawei, Zhao Baolei, Wang Jvcai, Chai Shujun, Zhou Enmin, Zhang Gaiping
College of Veterinary Medicine, Northwest A&F University, Yangling, 712100, China; Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, China.
Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, China.
Antiviral Res. 2017 Mar;139:146-152. doi: 10.1016/j.antiviral.2017.01.003. Epub 2017 Jan 4.
Porcine parvovirus (PPV) is a causative agent of reproductive failure in pregnant sows. Classical inactivated vaccine is extensively used to control PPV infection, but problems concerning safety, such as incomplete inactivation may occur. In this study, a novel subunit vaccine against PPV based on virus-like particles (VLPs) formed from the complete PPV VP2 protein expressed in a prokaryotic system with co-expressed chaperones is reported. The VLPs have a similar size, shape, and hemagglutination property to the PPV. Immunization with these VLPs stimulated the neutralization antibody and hemagglutination inhibition (HI) antibody responses in mice and guinea pigs. The lymphocyte proliferation response and cytokine secretion was also induced in immunized guinea pigs comparable to those immunized with PPV inactivated vaccine. In addition, immunization with VLPs also significantly reduced the PPV content in the spleen of guinea pigs 14 days after the challenge with intact virus. These studies suggest that PPV VLPs created as described here could be a potential candidate for vaccine development.
猪细小病毒（PPV）是妊娠母猪繁殖失败的病原体。经典灭活疫苗被广泛用于控制PPV感染，但可能会出现诸如灭活不完全等安全性问题。在本研究中，报道了一种基于在原核系统中表达的完整PPV VP2蛋白与共表达伴侣蛋白形成的病毒样颗粒（VLP）的新型抗PPV亚单位疫苗。这些VLP在大小、形状和血凝特性方面与PPV相似。用这些VLP免疫可刺激小鼠和豚鼠产生中和抗体和血凝抑制（HI）抗体反应。免疫豚鼠还诱导了淋巴细胞增殖反应和细胞因子分泌，与用PPV灭活疫苗免疫的豚鼠相当。此外，用VLP免疫还显著降低了豚鼠在用完整病毒攻击14天后脾脏中的PPV含量。这些研究表明，本文所述的PPV VLP可能是疫苗开发的潜在候选物。
