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一种展示日本脑炎病毒B细胞和T细胞表位的新型重组病毒样颗粒在小鼠和豚鼠中提供保护性免疫。

A Novel Recombinant Virus-Like Particles Displaying B and T Cell Epitopes of Japanese Encephalitis Virus Offers Protective Immunity in Mice and Guinea Pigs.

作者信息

Anwar Muhammad Naveed, Jiang Chunying, Di Di, Zhang Junjie, Guo Shuang, Wang Xin, Hameed Muddassar, Wahaab Abdul, Shao Donghua, Li Zongjie, Liu Ke, Li Beibei, Qiu Yafeng, Ma Zhiyong, Wei Jianchao

机构信息

Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China.

出版信息

Vaccines (Basel). 2021 Sep 2;9(9):980. doi: 10.3390/vaccines9090980.

DOI:10.3390/vaccines9090980
PMID:34579217
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8473392/
Abstract

Virus-like particles (VLPs) are non-replicative vectors for the delivery of heterologous epitopes and are considered one of the most potent inducers of cellular and humoral immune responses in mice and guinea pigs. In the present study, VLP-JEVe was constructed by the insertion of six Japanese encephalitis virus (JEV) envelope protein epitopes into different surface loop regions of PPV VP2 by the substitution of specific amino acid sequences without altering the assembly of the virus; subsequently, the protective efficacy of this VLP-JEVe was evaluated against JEV challenge in mice and guinea pigs. Mice immunized with the VLP-JEVe antigen developed high titers of neutralizing antibodies and 100% protection against lethal JEV challenge. The neutralizing and hemagglutination inhibition (HI) antibody responses were also induced in guinea pigs vaccinated with VLP-JEVe. In addition, immunization with VLP-JEVe in mice induced effective neutralizing antibodies and protective immunity against PPV (porcine parvovirus) challenge in guinea pigs. These studies suggest that VLP-JEVe produced as described here could be a potential candidate for vaccine development.

摘要

病毒样颗粒（VLPs）是用于递送异源表位的非复制型载体，被认为是小鼠和豚鼠中细胞免疫和体液免疫反应的最有效诱导剂之一。在本研究中，通过替换特定氨基酸序列，将六个日本脑炎病毒（JEV）包膜蛋白表位插入PPV VP2的不同表面环区域，构建了VLP-JEVe，且不改变病毒的组装；随后，评估了这种VLP-JEVe对小鼠和豚鼠JEV攻击的保护效果。用VLP-JEVe抗原免疫的小鼠产生了高滴度的中和抗体，并对致死性JEV攻击具有100%的保护作用。用VLP-JEVe接种的豚鼠也诱导了中和及血凝抑制（HI）抗体反应。此外，在小鼠中用VLP-JEVe免疫可诱导有效的中和抗体，并对豚鼠的PPV（猪细小病毒）攻击产生保护性免疫。这些研究表明，本文所述产生的VLP-JEVe可能是疫苗开发的潜在候选物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1358/8473392/c500885af8e4/vaccines-09-00980-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1358/8473392/d19b5e32ce93/vaccines-09-00980-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1358/8473392/0a8c1ba36299/vaccines-09-00980-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1358/8473392/10e6a1d3a2fe/vaccines-09-00980-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1358/8473392/b8d28f628fa3/vaccines-09-00980-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1358/8473392/ddbac5186ca2/vaccines-09-00980-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1358/8473392/c500885af8e4/vaccines-09-00980-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1358/8473392/d19b5e32ce93/vaccines-09-00980-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1358/8473392/0a8c1ba36299/vaccines-09-00980-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1358/8473392/10e6a1d3a2fe/vaccines-09-00980-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1358/8473392/b8d28f628fa3/vaccines-09-00980-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1358/8473392/ddbac5186ca2/vaccines-09-00980-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1358/8473392/c500885af8e4/vaccines-09-00980-g006.jpg

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