Giribabu Nelli, Reddy Pamanji Sreenivasula
Department of Zoology, Sri Venkateswara University, Tirupati 517 502, India.
Department of Zoology, Sri Venkateswara University, Tirupati 517 502, India.
Biomed Pharmacother. 2017 Mar;87:355-365. doi: 10.1016/j.biopha.2016.12.106. Epub 2017 Jan 5.
Di-n-butyl phthalate (DBP) widely spread industrial chemical that made drastic alteration in male reproductive system. The present study elucidates the protective role of testosterone on reproductive toxicity in prenatal DBP exposed adult male rats. Pregnant rats were injected with corn oil or 100 and 500mg/kg body weight of DBP on gestation day (GD) 1, 7 and 14. F1 male rats were weaned, injected with either testosterone or vehicle. On postnatal day (PND) 100 F1 adult male rats were cohabited with untreated female rats. Then rats were sacrificed and analyzed for other reproductive end points. Prenatal DBP exposed male rat testes, seminal vesicle weight, sperm count, motility, viability and HOS tail coiled sperm were significantly decreased with increased sperm morphological abnormalities. The levels of testicular 3β, 17βHSD, serum testosterone were significantly decreased with increased FSH, LH levels in experimental rats. The fertility studies revealed that increased pre, post-implantation losses and resorptions in normal females cohabited with experimental rats. Higher testicular LPO with lower SOD, CAT and GPx activity levels in experimental rats. Administration of testosterone to prenatal DBP treated male rats showed significant protection in above all parameters. In conclusions, testosterone deteriorates prenatal DBP induced reproductive and fertility toxicity by decreased oxidative stress and increased testicular antioxidant enzymes.
邻苯二甲酸二丁酯（DBP）是一种广泛传播的工业化学品，会对雄性生殖系统造成剧烈改变。本研究阐明了睾酮对产前暴露于DBP的成年雄性大鼠生殖毒性的保护作用。在妊娠第1天、第7天和第14天，给怀孕大鼠注射玉米油或100和500mg/kg体重的DBP。F1代雄性大鼠断奶后，注射睾酮或赋形剂。在出生后第100天，将F1代成年雄性大鼠与未处理的雌性大鼠同居。然后处死大鼠并分析其他生殖终点指标。产前暴露于DBP的雄性大鼠睾丸、精囊重量、精子计数、活力、存活率和低渗肿胀试验（HOS）尾部卷曲精子数量显著减少，精子形态异常增加。实验大鼠睾丸3β，17β-羟基类固醇脱氢酶（3β，17βHSD）水平、血清睾酮水平显著降低，促卵泡生成素（FSH）、促黄体生成素（LH）水平升高。生育力研究表明，与实验大鼠同居的正常雌性大鼠着床前、着床后损失及吸收增加。实验大鼠睾丸脂质过氧化（LPO）水平较高，超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GPx）活性水平较低。给产前经DBP处理的雄性大鼠注射睾酮后，上述所有参数均显示出显著的保护作用。总之，睾酮通过降低氧化应激和增加睾丸抗氧化酶，减轻了产前DBP诱导的生殖和生育毒性。
