Zhang Meng, Ishikawa Shintaro, Inagawa Tomoko, Ikemoto Hideshi, Guo Shiyu, Sunagawa Masataka, Hisamitsu Tadashi
Department of Physiology, School of Medicine, Showa University, Tokyo, Japan.
Department of Physiology, School of Medicine, Showa University, Tokyo, Japan
In Vivo. 2017 Jan 2;31(1):87-95. doi: 10.21873/invivo.11029.
To investigate the effect of mechanical stress on periostin and semaphorin-3A expression in a murine model of postmenopausal osteoporosis and in osteoblast-like MC3T3-E1 cells.
Female mice were divided into three groups and treated with a sham operation, ovariectomy (OVX) or OVX plus treadmill training (OVX+Run). After 10 weeks, tibias were used for histological analysis. MC3T3-E1 cells were burdened by mechanical stress using a centrifuge or were treated with periostin, and the production of biologically-active semaphorin-3A was examined in vitro.
In OVX+Run group tibias, the number of tartrate-resistant acid phosphatase-positive osteoclasts was lower than in the OVX group, and the expression of periostin and semaphorin-3A was higher. In MC3T3-E1 cells, centrifugal stress significantly increased periostin and semaphorin-3A mRNA expression. Treatment with periostin increased the semaphorin-3A level.
We speculate that mechanical load may increase periostin production in osteoblasts, and periostin may inhibit osteoclast differentiation by its effects on semaphorin-3A. Our results support the concept of a positive correlation between exercise and inhibition of osteoclasts in post-menopausal osteoporosis.
研究机械应力对绝经后骨质疏松小鼠模型和成骨样MC3T3-E1细胞中骨膜蛋白和信号素3A表达的影响。
将雌性小鼠分为三组，分别进行假手术、卵巢切除术（OVX）或卵巢切除术加跑步机训练（OVX+Run）。10周后，取胫骨进行组织学分析。使用离心机对MC3T3-E1细胞施加机械应力，或用骨膜蛋白处理细胞，并在体外检测生物活性信号素3A的产生。
在OVX+Run组小鼠的胫骨中，抗酒石酸酸性磷酸酶阳性破骨细胞的数量低于OVX组，骨膜蛋白和信号素3A的表达更高。在MC3T3-E1细胞中，离心应力显著增加了骨膜蛋白和信号素3A的mRNA表达。用骨膜蛋白处理可提高信号素3A水平。
我们推测机械负荷可能增加成骨细胞中骨膜蛋白的产生，并且骨膜蛋白可能通过其对信号素3A的作用抑制破骨细胞分化。我们的结果支持运动与绝经后骨质疏松症中破骨细胞抑制之间呈正相关的概念。
