Kovina A P, Petrova N V, Razin S V, Yarovaia O V
Biological Faculty, Moscow State University, Moscow, 119992 Russia.
Institute of Gene Biology, Russian Academy of Sciences, Moscow, 119334 Russia.
Mol Biol (Mosk). 2016 Nov-Dec;50(6):1020-1029. doi: 10.7868/S0026898416060112.
In warm-blooded vertebrates, the α- and β-globin genes are organized in domains of different types and are regulated in different fashion. In cold-blooded vertebrates and, in particular, the tropical fish Danio rerio, the α- and β-globin genes form two gene clusters. A major D. rerio globin gene cluster is in chromosome 3 and includes the α- and β-globin genes of embryonic-larval and adult types. The region upstream of the cluster contains c16orf35, harbors the main regulatory element (MRE) of the α-globin gene domain in warm-blooded vertebrates. In this study, transient transfection of erythroid cells with genetic constructs containing a reporter gene under the control of potential regulatory elements of the domain was performed to characterize the promoters of the embryonic-larval and adult α- and β-globin genes of the major cluster. Also, in the 5th intron of c16orf35 in Danio reriowas detected a functional analog of the warm-blooded vertebrate MRE. This enhancer stimulated activity of the promoters of both adult and embryonic-larval α- and β-globin genes.
在恒温脊椎动物中，α-珠蛋白基因和β-珠蛋白基因以不同类型的结构域形式组织，并以不同方式受到调控。在冷血脊椎动物中，特别是热带鱼斑马鱼，α-珠蛋白基因和β-珠蛋白基因形成两个基因簇。斑马鱼的一个主要珠蛋白基因簇位于3号染色体上，包括胚胎-幼体和成体类型的α-珠蛋白基因和β-珠蛋白基因。该基因簇上游区域含有c16orf35，其中含有恒温脊椎动物α-珠蛋白基因结构域的主要调控元件（MRE）。在本研究中，用含有在该结构域潜在调控元件控制下的报告基因的遗传构建体对红细胞进行瞬时转染，以表征主要基因簇中胚胎-幼体和成体α-珠蛋白基因和β-珠蛋白基因的启动子。此外，在斑马鱼c16orf35的第5内含子中检测到恒温脊椎动物MRE的功能类似物。这种增强子刺激了成体和胚胎-幼体α-珠蛋白基因和β-珠蛋白基因启动子的活性。
