Chetouani Asma, Follain Nadège, Marais Stéphane, Rihouey Christophe, Elkolli Meriem, Bounekhel Mahmoud, Benachour Djafer, Le Cerf Didier
Laboratory of Multiphase Polymeric Materials (LMPMP), Faculty of Technology, Setif-1 University, Setif 19000, Algeria.
Normandie Univ, UNIROUEN, INSA Rouen, CNRS, PBS, 76000 Rouen, France.
Int J Biol Macromol. 2017 Apr;97:348-356. doi: 10.1016/j.ijbiomac.2017.01.018. Epub 2017 Jan 5.
Pectin has been widely used in a variety of biomedical applications. In this study, it was modified with sodium periodate as an oxidant and characterized by physicochemical methods Periodate oxidation increased the contents of dialdehyde units and carboxyl groups in pectin, and a decrease in pectin viscosity was measured. The oxidization reaction led to a significant decrease in all values of molecular weight and size (Mn, Mw, [η] and Rh) as determined by size exclusion chromatography (SEC), which allowed the selection of the oxidized pectin to be added to chitosan. Chitosan-based films were characterized by infra-red spectroscopy (FTIR), X-ray diffractometry (XRD), and differential scanning calorimetry (DSC) measurements. Thermal behaviour studies demonstrated that interactions existed between chitosan and oxidized pectin. The haemolysis percentages of films were found to be less than 5%, which indicated their good blood compatibility. Finally, the antibacterial activity was clearly improved. Cross-linking reactions between pectin and chitosan through ionic bonds and amide bonds and between chitosan and oxidized pectin through Schiff base formation were evidenced, which opens the way to extend applications of these polysaccharides; notably, the biocompatibility and biodegradability of these new networks is convenient for pharmaceutical, biomedical or cosmetic applications.
果胶已广泛应用于各种生物医学领域。在本研究中，以高碘酸钠作为氧化剂对其进行改性，并采用物理化学方法对其进行表征。高碘酸盐氧化增加了果胶中二醛单元和羧基的含量，且测得果胶粘度降低。氧化反应导致通过尺寸排阻色谱法（SEC）测定的所有分子量和尺寸值（Mn、Mw、[η]和Rh）均显著降低，这使得可以选择将氧化果胶添加到壳聚糖中。基于壳聚糖的薄膜通过红外光谱（FTIR）、X射线衍射（XRD）和差示扫描量热法（DSC）测量进行表征。热行为研究表明壳聚糖与氧化果胶之间存在相互作用。发现薄膜的溶血率小于5%，这表明它们具有良好的血液相容性。最后，抗菌活性得到明显改善。证实了果胶与壳聚糖之间通过离子键和酰胺键发生交联反应，以及壳聚糖与氧化果胶之间通过席夫碱形成发生交联反应，这为扩展这些多糖的应用开辟了道路；值得注意的是，这些新网络的生物相容性和生物降解性便于用于制药、生物医学或化妆品应用。
