Galvão Stanley de S L, Monteiro Andrea de S, Siqueira Ezequias P, Bomfim Maria Rosa Q, Dias-Souza Marcus Vinícius, Ferreira Gabriella F, Denadai Angelo Márcio L, Santos Áquila R C, Lúcia Dos Santos Vera, de Souza-Fagundes Elaine M, Fernandes Elizabeth S, Monteiro-Neto Valério
Centro de Ciências da Saúde, Universidade CEUMA São Luís, Brazil.
Centro de Pesquisa René Rachou, Fundação Oswaldo Cruz-FIOCRUZ Belo Horizonte, Brazil.
Front Microbiol. 2016 Dec 21;7:2053. doi: 10.3389/fmicb.2016.02053. eCollection 2016.
is an important pathogen in opportunistic infections in humans. The increased incidence of antimicrobial-resistant isolates has highlighted the need for novel and more potent therapies against this microorganism. is known for presenting different compounds with diverse biological activities, such as anti-tumor and immunomodulatory activities. Although other species of the family display antimicrobial actions, this has not yet been reported for . Here, we investigated the antimicrobial activity of the ethyl acetate fraction (EAF) obtained from the leaf hydroalcoholic extract of . EAF was bactericidal against different strains of . EAF also presented with a time- and concentration-dependent effect on viability. Testing of different EAF sub-fractions showed that the sub-fraction 32-33 (SF32-33) was the most effective against . Analysis of the chemical constituents of SF32-33 demonstrated a high content of flavonoids. Incubation of this active sub-fraction with ATCC 27983 triggered an endothermic reaction, which was accompanied by an increased electric charge, suggesting a high binding of SF32-33 compounds to bacterial cell walls. Collectively, our results suggest that -derived compounds, especially flavonoids, may be useful for treating infections caused by .
是人类机会性感染中的一种重要病原体。抗菌耐药菌株发病率的增加凸显了针对这种微生物开发新型且更有效疗法的必要性。以呈现具有多种生物活性的不同化合物而闻名，例如抗肿瘤和免疫调节活性。尽管该家族的其他物种具有抗菌作用，但尚未见关于此的报道。在此，我们研究了从的叶水醇提取物中获得的乙酸乙酯馏分（EAF）的抗菌活性。EAF对不同菌株具有杀菌作用。EAF对的活力也呈现出时间和浓度依赖性效应。对不同EAF亚馏分的测试表明，亚馏分32 - 33（SF32 - 33）对最有效。对SF32 - 33化学成分的分析表明其黄酮类化合物含量很高。将这种活性亚馏分与ATCC 27983一起孵育引发了吸热反应，并伴随着电荷增加，这表明SF32 - 33化合物与细菌细胞壁有高度结合。总体而言，我们的结果表明，源自的化合物，尤其是黄酮类化合物，可能有助于治疗由引起的感染。
