Chakotiya Ankita Singh, Tanwar Ankit, Srivastava Pranay, Narula Alka, Sharma Rakesh Kumar
Division of CBRN Defence, Institute of Nuclear Medicine and Allied Sciences, Delhi - 110054, India.
Department of Biotechnology, Jamia Hamdard, Delhi - 110062, India.
Biomed Pharmacother. 2017 Jun;90:171-178. doi: 10.1016/j.biopha.2017.03.055. Epub 2017 Mar 27.
The prevalence of lung infection caused by Pseudomonas aeruginosa strains that are classified as multi-drug resistant has increased considerably and is mainly attributed to relative insufficiency of potent chemotherapeutic modalities. The present study was conducted to evaluate the antimicrobial activity of aquo-alcoholic extract of Glycyrrhiza glabra against the P. aeruginosa causing lung infection in Swiss albino mice. The study involves evaluation of lethal dose of P. aeruginosa in Swiss albino mice and analysis of disease manifestation that includes bacteremia, hypothermia, reduction in body weight and other parameters for 48h of infection. Physical manifestations of infected mice showed a significant decline in body temperature that is 29±0.57°C (at 48th h) from 38.81±0.33°C (0h) and 30% weight loss was observed at the end of the study. Further the efficacy of G. glabra extract against lung infection induced with the calculated lethal dose was evaluated by employing bacteremia, histopathology and radiological analysis. Bacterial burden showed that 2.30±0.02 Log10CFU/mL at day 7, a significant decline in the bacterial load as compared to day 1 when the bacterial burden was found to be 3.32±0.1 Log10CFU/mL. Histopathological results showed more diffuse and patchy accumulation of inflammatory cells within the alveolar space also the infiltrates were noted in all the lung section of infected mice. In treated animal group improved lung histology was seen with the exudates were less seen in D1 dose (20mg/kg) and disappeared in D2 dose (80mg/kg). The study clearly declares that the G. glabra extract is effective against lung infection caused by P. aeruginosa at dose of 80mg/kg. The LCMS results revealed that the extract contains Glycyrrhizin, Stigmasterol and Ergosterol, Licochalcone and Glabridin. The current study expected to further exploit the biomedical properties of this extract in the preparation of a potent regimen against such threatening pathogen.
被归类为多重耐药的铜绿假单胞菌菌株引起的肺部感染患病率显著增加，这主要归因于有效化疗方式相对不足。本研究旨在评估光果甘草水醇提取物对瑞士白化小鼠肺部感染铜绿假单胞菌的抗菌活性。该研究包括评估铜绿假单胞菌在瑞士白化小鼠中的致死剂量，并分析感染48小时内的疾病表现，包括菌血症、体温过低、体重减轻和其他参数。感染小鼠的身体表现显示体温显著下降，从38.81±0.33°C（0小时）降至29±0.57°C（第48小时），并且在研究结束时观察到体重减轻30%。此外，通过菌血症、组织病理学和放射学分析评估了光果甘草提取物对用计算出的致死剂量诱导的肺部感染的疗效。细菌载量显示，第7天为2.30±0.02 Log10CFU/mL，与第1天相比细菌载量显著下降，第1天细菌载量为3.32±0.1 Log10CFU/mL。组织病理学结果显示，肺泡腔内炎症细胞有更弥漫和片状的积聚，在感染小鼠的所有肺切片中也观察到浸润。在治疗动物组中，肺组织学得到改善，在D1剂量（20mg/kg）下渗出物较少，在D2剂量（80mg/kg）下渗出物消失。该研究明确表明，光果甘草提取物在80mg/kg剂量下对铜绿假单胞菌引起的肺部感染有效。液相色谱-质谱联用结果显示，该提取物含有甘草酸、豆甾醇和麦角甾醇、光甘草定和光甘草碱。本研究有望进一步开发该提取物的生物医学特性，以制备针对这种威胁性病原体的有效方案。
