Wang Yanyue, Wu Cuichen, Chen Tao, Sun Hao, Cansiz Sena, Zhang Liqin, Cui Cheng, Hou Weijia, Wu Yuan, Wan Shuo, Cai Ren, Liu Yuan, Sumerlin Brent, Zhang Xiaobing, Tan Weihong
Center for Research at Bio/Nano Interface, Department of Chemistry and Department of Physiology and Functional Genomics, Health Cancer Center, UF Genetics Institute and McKnight Brain Institute, University of Florida, Gainesville, Florida 32611-7200, United States.
Molecular Science and Biomedicine Laboratory, State Key Laboratory for Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, and Collaborative Research Center of Molecular Engineering for Theranostics, Hunan University, Changsha 410082, China.
Chem Sci. 2016;7(9):6041-6049. doi: 10.1039/C6SC00066E. Epub 2016 May 23.
DMFs are spherical DNA-diacyllipid nanostructures formed by hydrophobic effects between lipid tails coupled to single-stranded DNAs. Such properties as high cellular permeability, low critical micelle concentration (CMC) and facile fabrication facilitate intracellular imaging and drug delivery. While the basic properties of NFs have been amply described and tested, few studies have characterized the fundamental properties of DMFs with particular respect to aggregation number, dissociation constant and biostability. Therefore, to further explore their conformational features and enhanced stability in complex biological systems, we herein report a series of characterization studies. Static light scattering (SLS) demonstrated that DMFs possess greater DNA loading capacity when compared to other DNA-based nanostructures. Upon binding to complementary DNA (cDNA), DMFs showed excellent dissociation constants (K) and increased melting temperatures, as well as constant CMC (10 nM) independent of DNA length. DMFs also present significantly enhanced stability in aqueous solution with nuclease and cell lysate. These properties make DMFs ideal for versatile applications in bioanalysis and theranostics studies.
双分子膜（DMFs）是由与单链DNA偶联的脂质尾部之间的疏水作用形成的球形DNA - 二酰基脂质纳米结构。高细胞通透性、低临界胶束浓度（CMC）和易于制备等特性有助于细胞内成像和药物递送。虽然纳米纤维（NFs）的基本特性已得到充分描述和测试，但很少有研究特别针对聚集数、解离常数和生物稳定性来表征双分子膜的基本特性。因此，为了进一步探索它们在复杂生物系统中的构象特征和增强的稳定性，我们在此报告了一系列表征研究。静态光散射（SLS）表明，与其他基于DNA的纳米结构相比，双分子膜具有更大的DNA负载能力。与互补DNA（cDNA）结合后，双分子膜显示出优异的解离常数（K）和升高的解链温度，以及与DNA长度无关的恒定CMC（10 nM）。双分子膜在含有核酸酶和细胞裂解液的水溶液中也表现出显著增强的稳定性。这些特性使双分子膜成为生物分析和治疗诊断研究中广泛应用的理想选择。
