Tang C K, Vaze A, Rusling J F
Department of Chemistry, University of Connecticut, 55 North Eagleville Road, Storrs, Connecticut 06269-3060, USA.
Lab Chip. 2017 Jan 31;17(3):484-489. doi: 10.1039/c6lc01238h.
A low cost three-dimensional (3D) printed clear plastic microfluidic device was fabricated for fast, low cost automated protein detection. The unibody device features three reagent reservoirs, an efficient 3D network for passive mixing, and an optically transparent detection chamber housing a glass capture antibody array for measuring chemiluminescence output with a CCD camera. Sandwich type assays were built onto the glass arrays using a multi-labeled detection antibody-polyHRP (HRP = horseradish peroxidase). Total assay time was ∼30 min in a complete automated assay employing a programmable syringe pump so that the protocol required minimal operator intervention. The device was used for multiplexed detection of prostate cancer biomarker proteins prostate specific antigen (PSA) and platelet factor 4 (PF-4). Detection limits of 0.5 pg mL were achieved for these proteins in diluted serum with log dynamic ranges of four orders of magnitude. Good accuracy vs. ELISA was validated by analyzing human serum samples. This prototype device holds good promise for further development as a point-of-care cancer diagnostics tool.
制造了一种低成本的三维（3D）打印透明塑料微流控装置，用于快速、低成本的自动化蛋白质检测。该一体式装置具有三个试剂储存器、一个用于被动混合的高效3D网络，以及一个光学透明的检测室，该检测室容纳一个玻璃捕获抗体阵列，用于通过电荷耦合器件（CCD）相机测量化学发光输出。使用多标记检测抗体-聚辣根过氧化物酶（HRP = 辣根过氧化物酶）在玻璃阵列上构建夹心型检测法。在使用可编程注射泵的完全自动化检测中，总检测时间约为30分钟，因此该方案所需的操作人员干预最少。该装置用于多重检测前列腺癌生物标志物蛋白前列腺特异性抗原（PSA）和血小板因子4（PF-4）。在稀释血清中，这些蛋白质的检测限达到0.5 pg/mL，对数动态范围为四个数量级。通过分析人血清样本，验证了与酶联免疫吸附测定（ELISA）相比的良好准确性。作为一种即时癌症诊断工具，该原型装置具有进一步开发的良好前景。
