Garcia-Rey Sandra, Nielsen Jacob B, Nordin Gregory P, Woolley Adam T, Basabe-Desmonts Lourdes, Benito-Lopez Fernando
Microfluidics Cluster UPV/EHU, Analytical Microsystems & Materials for Lab-on-a-Chip (AMMa-LOAC) Group, Analytical Chemistry Department, University of the Basque Country UPV/EHU, 48940 Leioa, Spain.
Microfluidics Cluster UPV/EHU, BIOMICs Microfluidics Group, Lascaray Research Center, University of the Basque Country UPV/EHU, 01006 Vitoria-Gasteiz, Spain.
Polymers (Basel). 2022 Jun 22;14(13):2537. doi: 10.3390/polym14132537.
Additive manufacturing technology is an emerging method for rapid prototyping, which enables the creation of complex geometries by one-step fabrication processes through a layer-by-layer approach. The simplified fabrication achieved with this methodology opens the way towards a more efficient industrial production, with applications in a great number of fields such as biomedical devices. In biomedicine, blood is the gold-standard biofluid for clinical analysis. However, blood cells generate analytical interferences in many test procedures; hence, it is important to separate plasma from blood cells before analytical testing of blood samples. In this research, a custom-made resin formulation combined with a high-resolution 3D printing methodology were used to achieve a methodology for the fast prototype optimization of an operative plasma separation modular device. Through an iterative process, 17 different prototypes were designed and fabricated with printing times ranging from 5 to 12 min. The final device was evaluated through colorimetric analysis, validating this fabrication approach for the qualitative assessment of plasma separation from whole blood. The 3D printing method used here demonstrates the great contribution that this microfluidic technology will bring to the plasma separation biomedical devices market.
增材制造技术是一种用于快速成型的新兴方法，它能够通过逐层方法，利用一步制造工艺创建复杂的几何形状。这种方法实现的简化制造为更高效的工业生产开辟了道路，在生物医学设备等众多领域都有应用。在生物医学中，血液是临床分析的金标准生物流体。然而，血细胞在许多检测程序中会产生分析干扰；因此，在对血样进行分析测试之前，将血浆与血细胞分离很重要。在本研究中，一种定制的树脂配方与高分辨率3D打印方法相结合，用于实现一种对可操作血浆分离模块化设备进行快速原型优化的方法。通过迭代过程，设计并制造了17种不同的原型，打印时间从5分钟到12分钟不等。通过比色分析对最终设备进行了评估，验证了这种制造方法用于从全血中定性评估血浆分离的可行性。这里使用的3D打印方法证明了这种微流控技术将给血浆分离生物医学设备市场带来的巨大贡献。
