OleD Loki 作为三价胺和羟肟酸糖基化的催化剂。
OleD Loki as a Catalyst for Tertiary Amine and Hydroxamate Glycosylation.
机构信息
Center for Pharmaceutical Research and Innovation, College of Pharmacy, University of Kentucky, 789 South Limestone Street, Lexington, KY, 40536, USA.
Department of Chemistry, Rice University, P. O. Box 1892, MS 60, Houston, TX, 77251, USA.
出版信息
Chembiochem. 2017 Feb 16;18(4):363-367. doi: 10.1002/cbic.201600676. Epub 2017 Jan 9.
Abstract
We describe the ability of an engineered glycosyltransferase (OleD Loki) to catalyze the N-glycosylation of tertiary-amine-containing drugs and trichostatin hydroxamate glycosyl ester formation. As such, this study highlights the first bacterial model catalyst for tertiary-amine N-glycosylation and further expands the substrate scope and synthetic potential of engineered OleDs. In addition, this work could open the door to the discovery of similar capabilities among other permissive bacterial glycosyltransferases.
摘要
我们描述了一种工程化的糖基转移酶（OleD Loki）催化含叔胺药物的 N-糖基化和曲古抑菌素羟肟酸糖基酯形成的能力。因此，本研究突出了第一个细菌模型催化剂用于叔胺 N-糖基化，并进一步扩展了工程化 OleD 的底物范围和合成潜力。此外，这项工作可能为在其他许可的细菌糖基转移酶中发现类似的能力打开大门。
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