Kainulainen Veera, Tang Yurui, Spillmann Thomas, Kilpinen Susanne, Reunanen Justus, Saris Per E J, Satokari Reetta
Department of Veterinary Biosciences, Faculty of Veterinary Medicine, University of Helsinki, P.O. Box 66 (Agnes Sjöberginkatu 2), FI-00014, Helsinki, Finland.
Department of Food and Environmental Sciences, Faculty of Agriculture and Forestry, University of Helsinki, P.O. Box 56 (Viikinkaari 9), FI-00014, Helsinki, Finland.
BMC Microbiol. 2015 Jan 16;15(1):4. doi: 10.1186/s12866-014-0337-9.
For a good probiotic candidate, the abilities to adhere to intestinal epithelium and to fortify barrier function are considered to be crucial for colonization and functionality of the strain. The strain Lactobacillus acidophilus LAB20 was isolated from the jejunum of a healthy dog, where it was found to be the most pre-dominant lactobacilli. In this study, the adhesion ability of LAB20 to intestinal epithelial cell (IECs) lines, IECs isolated from canine intestinal biopsies, and to canine, porcine and human intestinal mucus was investigated. Further, we studied the ability of LAB20 to fortify the epithelial cell monolayer and to reduce LPS-induced interleukin (IL-8) release from enterocytes.
We found that LAB20 presented higher adhesion to canine colonic mucus as compared to mucus isolated from porcine colon. LAB20 showed adhesion to HT-29 and Caco-2 cell lines, and importantly also to canine IECs isolated from canine intestinal biopsies. In addition, LAB20 increased the transepithelial electrical resistance (TER) of enterocyte monolayers and thus strengthened the intestinal barrier function. The strain showed also anti-inflammatory capacity in being able to attenuate the LPS-induced IL-8 production of HT-29 cells.
In conclusion, canine indigenous strain LAB20 is a potential probiotic candidate for dogs adhering to the host epithelium and showing intestinal barrier fortifying and anti-inflammatory effects.
对于优良的益生菌候选菌株而言，黏附于肠道上皮和增强屏障功能的能力被认为对该菌株的定植及功能发挥至关重要。嗜酸乳杆菌LAB20菌株是从一只健康犬的空肠中分离得到的，在该空肠中它是最为优势的乳酸杆菌。在本研究中，我们调查了LAB20对肠道上皮细胞（IECs）系、从犬肠道活检组织中分离得到的IECs以及对犬、猪和人肠道黏液的黏附能力。此外，我们研究了LAB20增强上皮细胞单层结构以及减少脂多糖（LPS）诱导的肠细胞白细胞介素（IL - 8）释放的能力。
我们发现，与从猪结肠分离得到的黏液相比，LAB20对犬结肠黏液的黏附性更高。LAB20对HT - 29和Caco - 2细胞系有黏附性，重要的是，对从犬肠道活检组织中分离得到的犬IECs也有黏附性。此外，LAB20增加了肠细胞单层的跨上皮电阻（TER），从而增强了肠道屏障功能。该菌株还具有抗炎能力，能够减弱LPS诱导的HT - 29细胞IL - 8的产生。
总之，犬源菌株LAB20是一种潜在的犬用益生菌候选菌株，它能黏附于宿主上皮，具有增强肠道屏障及抗炎作用。
