仿生纺制嵌合蛛丝蛋白的人工蜘蛛丝。
Biomimetic spinning of artificial spider silk from a chimeric minispidroin.
机构信息
Department of Anatomy, Physiology and Biochemistry, Swedish University of Agricultural Sciences, Uppsala, Sweden.
Institute of Biological Sciences and Biotechnology, Donghua University, Shanghai, P.R. China.
出版信息
Nat Chem Biol. 2017 Mar;13(3):262-264. doi: 10.1038/nchembio.2269. Epub 2017 Jan 9.
Herein we present a chimeric recombinant spider silk protein (spidroin) whose aqueous solubility equals that of native spider silk dope and a spinning device that is based solely on aqueous buffers, shear forces and lowered pH. The process recapitulates the complex molecular mechanisms that dictate native spider silk spinning and is highly efficient; spidroin from one liter of bacterial shake-flask culture is enough to spin a kilometer of the hitherto toughest as-spun artificial spider silk fiber.
在此，我们展示了一种嵌合重组蜘蛛丝蛋白（丝氨酸），其水溶性与天然蜘蛛丝纺丝液相当，以及一种仅基于水缓冲液、剪切力和降低 pH 值的纺丝装置。该过程再现了决定天然蜘蛛丝纺丝的复杂分子机制，并且非常高效；从一升细菌摇瓶培养物中提取的丝氨酸足以纺出一千米迄今为止最坚韧的未经拉伸的人工蜘蛛丝纤维。
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