Siti Hawa Nordin, Kamisah Yusof, Nur Iliyani Mohd Ishak, Mohamed Suhaila, Jaarin Kamsiah
Department of Pharmacology, Faculty of Medicine, Universiti Kebangsaan Malaysia Medical Centre, Kuala Lumpur, Malaysia; Department of Basic Medical Sciences, Faculty of Medicine, Universiti Sultan Zainal Abidin, Terengganu, Malaysia.
Department of Pharmacology, Faculty of Medicine, Universiti Kebangsaan Malaysia Medical Centre, Kuala Lumpur, Malaysia.
Biomed Pharmacother. 2017 Mar;87:451-460. doi: 10.1016/j.biopha.2016.12.075. Epub 2017 Jan 6.
Prolonged consumption of repeatedly heated vegetable oil increases blood pressure. This study aimed to determine the effects of Citrus leaf extract, (CLE) on blood pressure, blood pressure-regulating enzymes and mediators, as well as aortic histomorphometry in heated palm oil induced-hypertension. Male Sprague Dawley rats (n=56) were divided into seven groups; control group was given normal diet and the other groups were fed with palm oil-enriched diet (15% w/w) either fresh (FPO), five-time-heated (5HPO) or ten-time-heated (10HPO) with or without CLE (0.15%, w/w) supplementation. CLE supplementation reduced the heated oil-raising effect of blood pressure, plasma TBARS, thromboxane and angiotensin-1 converting enzyme in 5HPO but not in 10HPO group. CLE increased serum heme oxygenase-1 in both 5HPO and 10HPO groups. CLE supplementation reduced the increase in aortic intima-media thickness, intima-media area and circumferential wall tension in 5HPO group but not in 10HPO group. These findings suggested that CLE supplementation reduces the blood pressure-raising effects of 5HPO and vascular damage, possibly through its antioxidant effect by modulating vasoactive mediators and blood pressure-regulating enzymes.
长期食用反复加热的植物油会导致血压升高。本研究旨在确定柑橘叶提取物（CLE）对加热棕榈油诱导的高血压大鼠的血压、血压调节酶和介质以及主动脉组织形态计量学的影响。将56只雄性Sprague Dawley大鼠分为七组；对照组给予正常饮食，其他组喂食富含棕榈油的饮食（15% w/w），分别为新鲜棕榈油（FPO）、五次加热的棕榈油（5HPO）或十次加热的棕榈油（10HPO），并补充或不补充CLE（0.15%，w/w）。补充CLE可降低5HPO组中加热油引起的血压、血浆丙二醛、血栓素和血管紧张素-1转换酶升高的作用，但对10HPO组无此作用。在5HPO组和10HPO组中，CLE均增加了血清血红素加氧酶-1。补充CLE可降低5HPO组主动脉内膜中层厚度、内膜中层面积和周向壁张力的增加，但对10HPO组无此作用。这些发现表明，补充CLE可能通过调节血管活性介质和血压调节酶的抗氧化作用，降低5HPO引起的血压升高作用和血管损伤。
