Henrissat B, Claeyssens M, Tomme P, Lemesle L, Mornon J P
Centre de Recherches sur les Macromolécules Végétales, CNRS, Grenoble, France.
Gene. 1989 Sep 1;81(1):83-95. doi: 10.1016/0378-1119(89)90339-9.
The amino acid sequences of 21 beta-glycanases have been compared by hydrophobic cluster analysis. Six families of cellulases have been identified on the basis of primary structure homology: (A) endoglucanases B, C and E of Clostridium thermocellum; endoglucanases of Erwinia chrysanthemi and Bacillus sp.; endoglucanase III of Trichoderma reesei; endoglucanase I of Schizophyllum commune; (B) cellobiohydrolase II of T. reesei; endoglucanases of Cellulomonas fimi and Streptomyces sp; (C) cellobiohydrolases I of T. reesei and of Phanerochaete chrysosporium; endoglucanase I of T. reesei; (D) endoglucanase A of C. thermocellum and an endoglucanase from Ce. uda; (E) endoglucanase D of C. thermocellum and an endoglucanase from Pseudomonas fluorescens; (F) xylanases of C. thermocellum and of Cryptococcus albidus and the cellobio-hydrolase of Ce. fimi. For each family, conserved potentially catalytic residues have have been listed and previous allocations of the active-site residues are evaluated in the light of the alignment of the amino acid sequences. A strong homology is also reported for the putative cellulose-binding domains of cellulases of Ce. fimi and of P. fluorescens.
通过疏水簇分析比较了21种β-聚糖酶的氨基酸序列。根据一级结构同源性鉴定出了六个纤维素酶家族：(A) 嗜热栖热放线菌的内切葡聚糖酶B、C和E；菊欧文氏菌和芽孢杆菌属的内切葡聚糖酶；里氏木霉的内切葡聚糖酶III；裂褶菌的内切葡聚糖酶I；(B) 里氏木霉的纤维二糖水解酶II；纤维单胞菌和链霉菌属的内切葡聚糖酶；(C) 里氏木霉和黄孢原毛平革菌的纤维二糖水解酶I；里氏木霉的内切葡聚糖酶I；(D) 嗜热栖热放线菌的内切葡聚糖酶A和来自 uda 纤维杆菌的一种内切葡聚糖酶；(E) 嗜热栖热放线菌的内切葡聚糖酶D和来自荧光假单胞菌的一种内切葡聚糖酶；(F) 嗜热栖热放线菌和浅白隐球酵母的木聚糖酶以及纤维杆菌的纤维二糖水解酶。对于每个家族，列出了保守的潜在催化残基，并根据氨基酸序列比对评估了活性位点残基的先前分配。还报道了纤维杆菌和荧光假单胞菌纤维素酶的假定纤维素结合结构域具有很强的同源性。
