Guangxi Key Laboratory of Electrochemical and Magnetochemical Function Materials, College of Chemistry and Bioengineering, Guilin University of Technology, Guangxi 541004, China.
Guangxi Key Laboratory of Electrochemical and Magnetochemical Function Materials, College of Chemistry and Bioengineering, Guilin University of Technology, Guangxi 541004, China.
Food Chem. 2017 May 15;223:89-95. doi: 10.1016/j.foodchem.2016.12.026. Epub 2016 Dec 16.
A novel continuous microwave-assisted enzymatic digestion (cMAED) method is proposed for the digestion of protein from Scomberomorus niphonius to obtain potential antioxidant peptides. In this study, bromelain was found to have a high capacity for the digestion of the Scomberomorus niphonius protein. The following cMAED conditions were investigated: protease species, microwave power, temperature, bromelain content, acidity of the substrate solution, and incubation time. At 400W, 40°C, 1500U·g bromelain, 20% substrate concentration, pH 6.0 and 5min incubation, the degree of hydrolysis and total antioxidant activity of the hydrolysates were 15.86% and 131.49μg·mL, respectively. The peptide analyses showed that eight of the potential antioxidant peptide sequences, which ranged from 502.32 to 1080.55Da with 4-10 amino acid residues, had features typical of well-known antioxidant proteins. Thus, the new cMAED method can be useful to obtain potential antioxidant peptides from protein sources, such as Scomberomorus niphonius.
一种新型的连续微波辅助酶解（cMAED）方法被提出，用于从蓝圆鲹中酶解蛋白质以获得潜在的抗氧化肽。在本研究中，发现菠萝蛋白酶对蓝圆鲹蛋白质的消化能力很强。研究了以下 cMAED 条件：蛋白酶种类、微波功率、温度、菠萝蛋白酶含量、底物溶液酸度和孵育时间。在 400W、40°C、1500U·g 菠萝蛋白酶、20%底物浓度、pH6.0 和 5min 孵育条件下，水解度和总抗氧化活性分别为 15.86%和 131.49μg·mL。肽分析表明，有八个潜在的抗氧化肽序列，分子量范围为 502.32-1080.55Da，含有 4-10 个氨基酸残基，具有典型的已知抗氧化蛋白的特征。因此，这种新的 cMAED 方法可以用于从蓝圆鲹等蛋白质来源中获得潜在的抗氧化肽。
