Peng Fei, Ren Xin, Du Bin, Chen Linan, Yu Zuoqing, Yang Yuedong
Hebei Key Laboratory of Active Components and Functions in Natural Products, Hebei Normal University of Science and Technology, Qinhuangdao 066004, China.
Engineering Research Center of Chestnut Industry Technology, Ministry of Education, Qinhuangdao 066004, China.
Foods. 2022 Jul 21;11(14):2161. doi: 10.3390/foods11142161.
In this study, soluble dietary fiber (SDF) and insoluble dietary fiber (IDF) were extracted from Pyrus ussuriensis Maxim pomace via three methods including enzymic extraction (EE), microwave-assisted enzymatic extraction (MEE), and three-phase partitioning (TPP). The effects of different extraction methods on the structure, physicochemical property, and functional activity of the extracted dietary fiber were evaluated. The results showed that different extraction methods had significant effects on the extraction yield, molecular weight distribution, thermal stability, antioxidant activity, and hypoglycemic activity in vitro, but resulted in no difference in the structure and composition of functional groups. It is noteworthy that SDF extracted by TPP has a more complex and porous structure, lower molecular weight, and higher thermal stability, as well as better physicochemical properties and in vitro hypoglycemic activity. IDF extracted by MEE showed the greatest water and oil holding capacity; the highest adsorption capacity for glucose, cholesterol, and nitrite ion; as well as the strongest inhibitory activity on α-amylase. These results suggest that PUP may be a source of cheap natural dietary fiber.
在本研究中，通过酶法提取（EE）、微波辅助酶法提取（MEE）和三相分离法（TPP）三种方法，从秋子梨果渣中提取可溶性膳食纤维（SDF）和不溶性膳食纤维（IDF）。评估了不同提取方法对所提取膳食纤维的结构、理化性质和功能活性的影响。结果表明，不同提取方法对提取率、分子量分布、热稳定性、抗氧化活性和体外降血糖活性有显著影响，但在官能团的结构和组成上没有差异值得注意的是，通过TPP提取的SDF具有更复杂且多孔的结构、较低的分子量和较高的热稳定性，以及更好的理化性质和体外降血糖活性。通过MEE提取的IDF表现出最大的持水和持油能力；对葡萄糖、胆固醇和亚硝酸根离子的吸附能力最高；以及对α-淀粉酶的抑制活性最强。这些结果表明，秋子梨果渣可能是廉价天然膳食纤维的来源。
