Komsta Łukasz, Stępkowska Barbara, Skibiński Robert
Department of Medicinal Chemistry, Faculty of Pharmacy, Medical University of Lublin, Jaczewskiego 4, 20-090 Lublin, Poland.
Department of Medicinal Chemistry, Faculty of Pharmacy, Medical University of Lublin, Jaczewskiego 4, 20-090 Lublin, Poland.
J Chromatogr A. 2017 Feb 3;1483:138-141. doi: 10.1016/j.chroma.2017.01.010. Epub 2017 Jan 4.
The eluotropic strength on thin-layer silica plates was investigated for 20 chromatographic grade solvents available in current market. 35 model compounds were used as test subjects in the investigation. The use of modern mixture screening design allowed to estimate each solvent as a separate elution coefficient with an acceptable error of estimation (0.0913 of R value). Additional bootstrapping technique was used to check the distribution and uncertainty of eluotropic estimates, proving very similar confidence intervals to linear regression. Principal component analysis proved that the only one parameter (mean eluotropic strength) is satisfactory to describe the solvent property, as it explains almost 90% of variance of retention. The obtained eluotropic data can be good appendix to earlier published results and their values can be interpreted in context of R differences.
针对目前市场上可得的20种色谱级溶剂，研究了其在薄层硅胶板上的洗脱强度。在该研究中，使用35种模型化合物作为测试对象。采用现代混合筛选设计能够以可接受的估计误差（R值为0.0913）将每种溶剂估计为单独的洗脱系数。使用额外的自助法技术来检查洗脱强度估计值的分布和不确定性，结果表明其置信区间与线性回归非常相似。主成分分析证明，仅一个参数（平均洗脱强度）就足以描述溶剂性质，因为它解释了几乎90%的保留方差。所获得的洗脱强度数据可以很好地补充早期发表的结果，并且其值可以根据R差异来解释。
