• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
Snapshot Profiling of the Antileishmanial Potency of Lead Compounds and Drug Candidates against Intracellular Leishmania donovani Amastigotes, with a Focus on Human-Derived Host Cells.先导化合物和候选药物对细胞内杜氏利什曼原虫无鞭毛体的抗利什曼活性的快照分析，重点关注人源宿主细胞。
Antimicrob Agents Chemother. 2017 Feb 23;61(3). doi: 10.1128/AAC.01228-16. Print 2017 Mar.
2
A parasite rescue and transformation assay for antileishmanial screening against intracellular Leishmania donovani amastigotes in THP1 human acute monocytic leukemia cell line.一种用于在THP1人急性单核细胞白血病细胞系中针对细胞内杜氏利什曼原虫无鞭毛体进行抗利什曼原虫筛选的寄生虫拯救与转化试验。
J Vis Exp. 2012 Dec 30(70):4054. doi: 10.3791/4054.
3
N-(2-hydroxypropyl)methacrylamide-amphotericin B (HPMA-AmB) copolymer conjugates as antileishmanial agents.N-（2-羟丙基）甲基丙烯酰胺-两性霉素B（HPMA-AmB）共聚物缀合物作为抗利什曼原虫药物。
Int J Antimicrob Agents. 2009 May;33(5):441-8. doi: 10.1016/j.ijantimicag.2008.10.013. Epub 2008 Dec 20.
4
In vitro interactions between sitamaquine and amphotericin B, sodium stibogluconate, miltefosine, paromomycin and pentamidine against Leishmania donovani.在体外用疟喹与两性霉素 B、葡萄糖酸锑钠、米替福新、巴龙霉素和喷他脒抗杜氏利什曼原虫的相互作用。
J Antimicrob Chemother. 2011 Apr;66(4):850-4. doi: 10.1093/jac/dkq542. Epub 2011 Feb 3.
5
Pentostam induces resistance to antimony and the preservative chlorocresol in Leishmania donovani promastigotes and axenically grown amastigotes.喷他脒可诱导杜氏利什曼原虫前鞭毛体和体外培养的无鞭毛体对锑和防腐剂氯甲酚产生抗性。
Antimicrob Agents Chemother. 1997 May;41(5):1064-8. doi: 10.1128/AAC.41.5.1064.
6
In vitro susceptibilities of Leishmania donovani promastigote and amastigote stages to antileishmanial reference drugs: practical relevance of stage-specific differences.杜氏利什曼原虫前鞭毛体和无鞭毛体阶段对抗利什曼原虫参考药物的体外敏感性：阶段特异性差异的实际意义
Antimicrob Agents Chemother. 2009 Sep;53(9):3855-9. doi: 10.1128/AAC.00548-09. Epub 2009 Jun 22.
7
In vitro and in vivo pharmacodynamics of three novel antileishmanial lead series.三种新型抗利什曼原虫先导化合物的体外和体内药效学研究。
Int J Parasitol Drugs Drug Resist. 2018 Apr;8(1):81-86. doi: 10.1016/j.ijpddr.2018.01.006. Epub 2018 Jan 31.
8
Growth of Leishmania donovani amastigotes in the continuous human macrophage cell line U937: studies of drug efficacy and metabolism.杜氏利什曼原虫无鞭毛体在人巨噬细胞系U937中的生长：药物疗效及代谢研究
J Infect Dis. 1986 Aug;154(2):323-7. doi: 10.1093/infdis/154.2.323.
9
Stage-specific activity of pentavalent antimony against Leishmania donovani axenic amastigotes.五价锑对杜氏利什曼原虫体外无鞭毛体的阶段特异性活性。
Antimicrob Agents Chemother. 1999 Feb;43(2):278-82. doi: 10.1128/AAC.43.2.278.
10
Development of luciferase expressing Leishmania donovani axenic amastigotes as primary model for in vitro screening of antileishmanial compounds.表达荧光素酶的杜氏利什曼原虫无鞭毛体的构建及其作为抗利什曼原虫化合物体外筛选的初步模型。
Curr Microbiol. 2012 Dec;65(6):696-700. doi: 10.1007/s00284-012-0209-1. Epub 2012 Sep 4.

引用本文的文献

1
Free Radical Production Induced by Nitroimidazole Compounds Lead to Cell Death in Amastigotes.硝咪唑类化合物诱导的自由基产生导致无鞭毛体细胞死亡。
Molecules. 2024 Aug 26;29(17):4041. doi: 10.3390/molecules29174041.
2
Combination therapy using nitro compounds improves the efficacy of experimental Chagas disease treatment.联合使用硝基化合物的治疗方法提高了实验性恰加斯病治疗的疗效。
Parasitology. 2021 Sep;148(11):1320-1327. doi: 10.1017/S0031182021001001. Epub 2021 Jun 18.
3
Novel 2D and 3D Assays to Determine the Activity of Anti-Leishmanial Drugs.用于测定抗利什曼原虫药物活性的新型二维和三维检测方法。
Microorganisms. 2020 Jun 1;8(6):831. doi: 10.3390/microorganisms8060831.
4
Infectivity and Drug Susceptibility Profiling of Different -Host Cell Combinations.不同宿主细胞组合的感染性和药敏分析
Pathogens. 2020 May 20;9(5):393. doi: 10.3390/pathogens9050393.
5
Techniques to study phagocytosis and uptake of Leishmania tarentolae by J774 macrophages.研究J774巨噬细胞对热带利什曼原虫的吞噬作用和摄取的技术。
Exp Parasitol. 2019 Feb;197:57-64. doi: 10.1016/j.exppara.2019.01.012. Epub 2019 Jan 21.
6
Impact of primary mouse macrophage cell types on Leishmania infection and in vitro drug susceptibility.原代小鼠巨噬细胞类型对利什曼原虫感染及体外药物敏感性的影响。
Parasitol Res. 2018 Nov;117(11):3601-3612. doi: 10.1007/s00436-018-6059-4. Epub 2018 Aug 23.

本文引用的文献

1
A Replicative In Vitro Assay for Drug Discovery against Leishmania donovani.一种用于发现抗杜氏利什曼原虫药物的体外复制分析方法。
Antimicrob Agents Chemother. 2016 May 23;60(6):3524-32. doi: 10.1128/AAC.01781-15. Print 2016 Jun.
2
Functional Validation of ABCA3 as a Miltefosine Transporter in Human Macrophages: IMPACT ON INTRACELLULAR SURVIVAL OF LEISHMANIA (VIANNIA) PANAMENSIS.ABCA3作为米替福新转运体在人巨噬细胞中的功能验证：对巴拿马利什曼原虫（维阿尼亚种）细胞内存活的影响
J Biol Chem. 2016 Apr 29;291(18):9638-47. doi: 10.1074/jbc.M115.688168. Epub 2016 Feb 22.
3
Leishmania survival in the macrophage: where the ends justify the means.利什曼原虫在巨噬细胞中的存活：只要目的正当，可以不择手段。
Curr Opin Microbiol. 2015 Aug;26:32-40. doi: 10.1016/j.mib.2015.04.007. Epub 2015 May 17.
4
Nitroimidazo-oxazole compound DNDI-VL-2098: an orally effective preclinical drug candidate for the treatment of visceral leishmaniasis.硝咪唑并恶唑类化合物 DNDI-VL-2098：一种治疗内脏利什曼病的有效口服前临床候选药物。
J Antimicrob Chemother. 2015 Feb;70(2):518-27. doi: 10.1093/jac/dku422. Epub 2014 Nov 10.
5
Leishmania panamensis infection and antimonial drugs modulate expression of macrophage drug transporters and metabolizing enzymes: impact on intracellular parasite survival.感染泛美利什曼原虫和锑制剂调节巨噬细胞药物转运体和代谢酶的表达：对细胞内寄生虫存活的影响。
J Antimicrob Chemother. 2014 Jan;69(1):139-49. doi: 10.1093/jac/dkt334. Epub 2013 Aug 24.
6
The R enantiomer of the antitubercular drug PA-824 as a potential oral treatment for visceral Leishmaniasis.抗结核药物 PA-824 的 R 对映异构体作为治疗内脏利什曼病的潜在口服药物。
Antimicrob Agents Chemother. 2013 Oct;57(10):4699-706. doi: 10.1128/AAC.00722-13. Epub 2013 Jul 15.
7
High content analysis of primary macrophages hosting proliferating Leishmania amastigotes: application to anti-leishmanial drug discovery.原代巨噬细胞中增殖利什曼原虫的高通量分析：在抗利什曼原虫药物发现中的应用。
PLoS Negl Trop Dis. 2013 Apr 4;7(4):e2154. doi: 10.1371/journal.pntd.0002154. Print 2013.
8
Comparison of a high-throughput high-content intracellular Leishmania donovani assay with an axenic amastigote assay.高通量高内涵细胞内利什曼原虫检测与无细胞内鞭毛体检测的比较。
Antimicrob Agents Chemother. 2013 Jul;57(7):2913-22. doi: 10.1128/AAC.02398-12. Epub 2013 Apr 9.
9
Oxygen tension modulates differentiation and primary macrophage functions in the human monocytic THP-1 cell line.氧张力调节人单核细胞 THP-1 细胞系的分化和原代巨噬细胞功能。
PLoS One. 2013;8(1):e54926. doi: 10.1371/journal.pone.0054926. Epub 2013 Jan 23.
10
Antimony-resistant but not antimony-sensitive Leishmania donovani up-regulates host IL-10 to overexpress multidrug-resistant protein 1.耐锑但不敏感的杜氏利什曼原虫上调宿主白细胞介素 10 以过表达多药耐药蛋白 1。
Proc Natl Acad Sci U S A. 2013 Feb 12;110(7):E575-82. doi: 10.1073/pnas.1213839110. Epub 2013 Jan 22.

先导化合物和候选药物对细胞内杜氏利什曼原虫无鞭毛体的抗利什曼活性的快照分析，重点关注人源宿主细胞。

Snapshot Profiling of the Antileishmanial Potency of Lead Compounds and Drug Candidates against Intracellular Leishmania donovani Amastigotes, with a Focus on Human-Derived Host Cells.

作者信息

Koniordou Markela, Patterson Stephen, Wyllie Susan, Seifert Karin

机构信息

Faculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, United Kingdom.

School of Life Sciences, University of Dundee, Dundee, United Kingdom.

出版信息

Antimicrob Agents Chemother. 2017 Feb 23;61(3). doi: 10.1128/AAC.01228-16. Print 2017 Mar.

DOI:10.1128/AAC.01228-16
PMID:28069646
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5328536/
Abstract

This study characterized the potencies of antileishmanial agents against intracellular amastigotes in primary human macrophages, obtained with or without CD14-positive monocyte enrichment, phorbol 12-myristate 13-acetate (PMA)-differentiated THP-1 cells, and mouse peritoneal exudate macrophages (PEMs). Host cell-dependent potency was confirmed for pentavalent and trivalent antimony. Fexinidazole was inactive against intracellular amastigotes across the host cell panel. Fexinidazole sulfone, ()-PA-824, ()-PA-824, and VL-2098 displayed similar potency in all of the host cells tested.

摘要

本研究表征了抗利什曼原虫药物对原代人巨噬细胞内无鞭毛体的效力，这些原代人巨噬细胞通过有无CD14阳性单核细胞富集获得，佛波醇12-肉豆蔻酸酯13-乙酸酯（PMA）分化的THP-1细胞，以及小鼠腹腔渗出巨噬细胞（PEMs）。证实了五价和三价锑的宿主细胞依赖性效力。非昔硝唑对整个宿主细胞组中的细胞内无鞭毛体无活性。非昔硝唑砜、()-PA-824、()-PA-824和VL-2098在所有测试的宿主细胞中显示出相似的效力。