Looker D L, Martinez S, Horton J M, Marr J J
J Infect Dis. 1986 Aug;154(2):323-7. doi: 10.1093/infdis/154.2.323.
We have developed a simple and reproducible system for infecting a human macrophage cell line (U937) with stationary-phase Leishmania donovani promastigotes. Four days after infection, greater than 90% of the promastigotes had transformed to amastigotes. The antileishmanial agents allopurinol riboside, formycin B, 9-deazainosine, and sodium stibogluconate effectively inhibited the growth of L. donovani amastigotes in this cell line. To study the capability of amastigotes in the U937 cell line to carry out biochemical reactions that could be monitored experimentally, we incubated the cells with radiolabeled 9-deazainosine. This purine analogue underwent metabolism in the amastigote phase similar to that occurring in the promastigote phase. This cell line should be useful for studies of parasite maturation and differentiation, parasite-human interactions, and antiparasitic drugs.
我们开发了一种简单且可重复的系统，用于用静止期杜氏利什曼原虫前鞭毛体感染人巨噬细胞系（U937）。感染后四天，超过90%的前鞭毛体已转化为无鞭毛体。抗利什曼原虫药物别嘌呤醇核糖苷、间型霉素B、9-去氮肌苷和葡萄糖酸锑钠有效抑制了该细胞系中杜氏利什曼原虫无鞭毛体的生长。为了研究U937细胞系中的无鞭毛体进行可通过实验监测的生化反应的能力，我们用放射性标记的9-去氮肌苷孵育细胞。这种嘌呤类似物在无鞭毛体阶段的代谢与在前鞭毛体阶段发生的代谢相似。该细胞系应有助于寄生虫成熟和分化、寄生虫与人类相互作用以及抗寄生虫药物的研究。
