Moulana Zahra, Roushan Mohammad Reza Hasanjani, Marashi Seyed Mahmoud Amin
Ph.D. in Biomedical Sciences, Infectious Diseases and Tropical Medicine Research Center, Babol University of Medical Sciences, Babol, Iran.
Professor of Infectious Diseases, Infectious Diseases and Tropical Medicine Research Center, Babol University of Medical Sciences, Babol, Iran.
Electron Physician. 2016 Nov 25;8(11):3222-3227. doi: 10.19082/3222. eCollection 2016 Nov.
Brucellosis is a worldwide zoonosis and a significant cause of loss of health in humans and animals. Traditionally, classic diagnosis is carried out by isolation of , which is time-consuming, technically challenging and potentially dangerous. The aim of this study was to expand a molecular test that would be used for the develop detection of in a single reaction with high sensitivity and specificity, by targeting IS711element.
This study was carried out from 2015 to 2016 at the Ayatolla Rohani hospital in Babol, Iran. The present study was designed to develop PCR assay, based on IS711 gene for rapid diagnosis of . and immediate detection of , with high sensitivity and specificity. Four pairs of oligo-nucleotide primers with sizes of 547, 403, 291 and 127bp respectively, were planned to exclusively amplify the targeted genes of Brucella species.
Our results show that, five PCR primers set up, would be helpful in amplifying the DNAs from the genus with high specificity and sensitivity so it can be 12 fg, for species to provide a valuable tool for diagnosis.
This method can be more useful than serological and biochemical tests and in addition, this reduces the number of required tests more rapidly and economically.
布鲁氏菌病是一种全球性人畜共患病，是人类和动物健康损失的重要原因。传统上，经典诊断是通过分离[具体内容缺失]来进行的，这既耗时、技术上具有挑战性，又有潜在危险。本研究的目的是扩展一种分子检测方法，该方法通过靶向IS711元件，可在单一反应中用于高灵敏度和特异性地检测[具体内容缺失]。
本研究于2015年至2016年在伊朗巴博勒的阿亚图拉·罗哈尼医院进行。本研究旨在开发基于IS711基因的PCR检测方法，用于快速诊断[具体内容缺失]，并以高灵敏度和特异性即时检测[具体内容缺失]。设计了四对寡核苷酸引物，大小分别为547、403、291和127bp，专门用于扩增布鲁氏菌属的目标基因。
我们的结果表明，所建立的五组PCR引物有助于以高特异性和灵敏度扩增布鲁氏菌属的DNA，其灵敏度可达12fg，为[具体内容缺失]物种的诊断提供了一种有价值的工具。
该方法可能比血清学和生化检测更有用，此外，它能更快速、经济地减少所需检测的数量。
