Adabi Maryam, Karami Manoochehr, Keramat Fariba, Alikhani Mohammad Yousef, Bakhtiari Somaye
Brucellosis Research Center, Hamadan University of Medical Sciences, Hamadan, Iran.
Health Sciences Research Center, Hamadan University of Medical Sciences, Hamadan, Iran.
Iran J Microbiol. 2021 Jun;13(3):319-324. doi: 10.18502/ijm.v13i3.6394.
is an intracellular pathogen that causes brucellosis in humans and animals. This study aimed to assess the results of brucellosis seroprevalence among participants of the Famenin brucellosis cohort with molecular investigation technique and determine -approved species.
Following the first phase of the Famenin brucellosis cohort in 2016 which investigated the seroprevalence of brucellosis among 2367 participants in Famenin city, a total of 575 people including all seropositive and some seronegative people were examined again by wright serological tests in 2019. The PCR assay was accomplished on all cases that have wright titers ≥ 1/20 for tracing DNA using BCSP31 target gene and IS711 locus.
Out of 575 studied cases, 145 people had wright titers ≥ 1/20. The PCR reactions of these 145 blood samples were positive in 63/145 (43.44%) tested samples using primers (B4/B5) for genus detection. In the second PCR assay using specific-primers for and , 18/63 (28.57%) of the samples were diagnosed as , and 18/63 (28.57%) were diagnosed as
In this study, using the selected specific genes for the diagnosis of in the genus and species levels, the PCR technique was evaluated as a promising method for the rapid and safe detection of brucellosis besides the serological test for more accurate detection of brucellosis especially in cases that are not definitive.
布鲁氏菌是一种细胞内病原体，可导致人类和动物患布鲁氏菌病。本研究旨在通过分子检测技术评估法门宁布鲁氏菌病队列参与者中布鲁氏菌病血清流行率的结果，并确定布鲁氏菌的批准种属。
2016年法门宁布鲁氏菌病队列的第一阶段调查了法门宁市2367名参与者中布鲁氏菌病的血清流行率，2019年对包括所有血清阳性和部分血清阴性的5
