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豚鼠耳蜗CO激光造孔损伤机制分析

Analysis of the Damage Mechanism Related to CO Laser Cochleostomy on Guinea Pig Cochlea.

作者信息

Liu Xiang, Qian Xiao-Qing, Ma Rui, Chi Fang-Lu, Ren Dong-Dong

机构信息

Department of Otology & Skull Base Surgery, EYE & ENT Hospital of Fudan University, Shanghai 200031, China; Shanghai Clinical Medical Center of Hearing Medicine, Shanghai 200031, China; Key Laboratory of Hearing Medicine, Ministry of Health, Shanghai 200031, China.

Shanghai Clinical Medical Center of Hearing Medicine, Shanghai 200031, China; Key Laboratory of Hearing Medicine, Ministry of Health, Shanghai 200031, China; Department of Research Center, EYE & ENT Hospital of Fudan University, Shanghai 200031, China.

出版信息

Neural Plast. 2016;2016:5982397. doi: 10.1155/2016/5982397. Epub 2016 Dec 14.

DOI:10.1155/2016/5982397
PMID:28070426
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5192333/
Abstract

Different types of lasers have been used in inner ear surgery. Therefore, it is of the utmost importance to avoid damage to the inner ear (e.g., hyperthermia and acoustic effects) caused by the use of such lasers. The aim of this study was to use a high powered fibre-enabled CO laser (10 W, 606 J/cm) to perform cochleostomies on guinea pig cochlea and to investigate the possible laser-induced damage mechanisms. The temperature changes in the round window membrane, auditory evoked brainstem response, and morphological of the hair cells were measured and recorded before and after laser application. All of the outcomes differed in comparison with the control group. A rise in temperature and subsequent increased hearing loss were observed in animals that underwent surgery with a 10 W CO laser. These findings correlated with increased injury to the cochlear ultrastructure and a higher positive expression of E-cadherin and -catenin in the damaged organ of Corti. We assume that enhanced cell-cell adhesion and the activated -catenin-related canonical Wnt-signalling pathway may play a role in the protection of the cochlea to prevent further damage.

摘要

不同类型的激光已被用于内耳手术。因此，避免因使用此类激光对内耳造成损伤（如热疗和声效应）至关重要。本研究的目的是使用高功率光纤CO激光（10 W，606 J/cm）对豚鼠耳蜗进行蜗窗造孔，并研究可能的激光诱导损伤机制。在激光照射前后测量并记录圆窗膜的温度变化、听觉脑干诱发电位以及毛细胞的形态。所有结果与对照组相比均有差异。在接受10 W CO激光手术的动物中观察到温度升高以及随后听力损失增加。这些发现与耳蜗超微结构损伤增加以及受损柯蒂氏器中E-钙黏蛋白和β-连环蛋白的阳性表达升高相关。我们推测增强的细胞间黏附以及激活的β-连环蛋白相关的经典Wnt信号通路可能在保护耳蜗以防止进一步损伤中发挥作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98f5/5192333/5ab74702c6de/NP2016-5982397.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98f5/5192333/e31aacfced0c/NP2016-5982397.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98f5/5192333/1ec3bee8d5ce/NP2016-5982397.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98f5/5192333/c973417cd362/NP2016-5982397.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98f5/5192333/5ab74702c6de/NP2016-5982397.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98f5/5192333/e31aacfced0c/NP2016-5982397.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98f5/5192333/1ec3bee8d5ce/NP2016-5982397.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98f5/5192333/c973417cd362/NP2016-5982397.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98f5/5192333/5ab74702c6de/NP2016-5982397.004.jpg

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本文引用的文献

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Mol Med Rep. 2014 Jul;10(1):15-20. doi: 10.3892/mmr.2014.2202. Epub 2014 Apr 30.
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First experience with the fiber-enabled CO2 laser in stapes surgery and a comparison with the "one-shot" technique.纤维激光在镫骨手术中的首次应用及与“单次击发”技术的比较
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IR laser-induced perturbations of the voltage-dependent solute carrier protein SLC26a5.
IR 激光诱导电压依赖性溶质载体蛋白 SLC26a5 的变化。
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Comparison of KTP, Thulium, and CO2 laser in stapedotomy using specialized visualization techniques: thermal effects.比较 KTP、铥和二氧化碳激光在使用专业可视化技术的镫骨切开术中的应用：热效应。
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Reliability and efficacy of a new CO2 laser hollow fiber: a prospective study of 39 patients.新型二氧化碳激光空心光纤的可靠性和疗效：39 例患者的前瞻性研究。
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