Chen Li, Wang Ting, Chen Guanjun, Wang Nuojin, Gui Li, Dai Fang, Fang Zhaohui, Zhang Qiu, Lu Yunxia
Clinical Laboratory, Anhui Provincial Hospital, Hefei, Anhui, 230001, China.
Endocrinology Department, First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, 230022, China.
Endocrine. 2017 Mar;55(3):773-785. doi: 10.1007/s12020-016-1212-2. Epub 2017 Jan 9.
This study aimed to determine whether resveratrol treatment alleviates endoplasmic reticulum stress and changes the expression of adipokines in adipose tissues and cells.
8-week-old male C57BL/6 mice were fed a high-calorie diet (HCD group) or high-calorie diet supplemented with resveratrol (high-calorie diet + resveratrol group) for 3 months. Insulin resistance, serum lipids and proinflammatory indices, the size and inflammatory cell infiltration in subcutaneous and visceral adipose tissues were analyzed. The gene expressions of endoplasmic reticulum stress, adipokines, and inflammatory cytokines were determined. The induced mature 3T3-L1 cells were pretreated with resveratrol and then palmitic acid, and the gene expressions of endoplasmic reticulum stress, adipokines, and inflammatory cytokines were determined.
Subcutaneous and visceral adipose tissues in the high-calorie diet-fed mice exhibited adipocyte hypertrophy, inflammatory activation, and endoplasmic reticulum stress. Resveratrol alleviated high-calorie diet-induced insulin resistance and endoplasmic reticulum stress, increased expression of SIRT1, and reversed expression of adipokines in varying degrees in both subcutaneous and visceral adipose tissues. The effects of resveratrol on palmitic acid-treated adipocytes were similar to those shown in the tissues.
Resveratrol treatment obviously reversed adipocyte hypertrophy and insulin resistance by attenuating endoplasmic reticulum stress and inflammation, thus increasing the expression of SIRT1 and inverting the expression of adipokines in vivo and in vitro.
本研究旨在确定白藜芦醇治疗是否能减轻内质网应激，并改变脂肪组织和细胞中脂肪因子的表达。
将8周龄雄性C57BL/6小鼠分为两组，一组给予高热量饮食（HCD组），另一组给予补充白藜芦醇的高热量饮食（高热量饮食+白藜芦醇组），持续3个月。分析胰岛素抵抗、血脂和促炎指标，以及皮下和内脏脂肪组织的大小和炎症细胞浸润情况。测定内质网应激、脂肪因子和炎性细胞因子的基因表达。将诱导成熟的3T3-L1细胞先用白藜芦醇预处理，然后用棕榈酸处理，测定内质网应激、脂肪因子和炎性细胞因子的基因表达。
高热量饮食喂养的小鼠皮下和内脏脂肪组织出现脂肪细胞肥大、炎症激活和内质网应激。白藜芦醇减轻了高热量饮食诱导的胰岛素抵抗和内质网应激，增加了SIRT1的表达，并在皮下和内脏脂肪组织中不同程度地逆转了脂肪因子的表达。白藜芦醇对棕榈酸处理的脂肪细胞的作用与在组织中观察到的作用相似。
白藜芦醇治疗通过减轻内质网应激和炎症，明显逆转了脂肪细胞肥大和胰岛素抵抗，从而增加了SIRT1的表达，并在体内和体外逆转了脂肪因子的表达。
