Yoon Jung Jae, Sohn Eun Jung, Kim Jung Hyo, Seo Jai Wha, Kim Sung-Hoon
College of Korean Medicine, Kyung Hee University, 1 Hoegidong, Dongdaemungu, Seoul, 131-701, Korea.
Chosun Nursing College, Gwang-Ju, 501-759, Korea.
Phytother Res. 2017 Mar;31(3):418-424. doi: 10.1002/ptr.5763. Epub 2017 Jan 9.
Although Kaejadan (KJD), an herbal cocktail of three medicinal plants (Lithospermum erythrorhizon, Cinnamomum loureirii, and Salvia miltiorrhiza), has been traditionally used for the treatment of rheumatoid arthritis, its scientific evidence is not fully understood. Hence, we investigated antiinflammatory and analgesic mechanism of KJD in vivo and in vitro. Kaejadan suppressed the number of writhing responses in mice treated by acetic acid and showed antinociceptive effect by tail-flick test. Kaejadan abrogated serotonin or carrageenan or Freund's complete adjuvant (FCA)-induced paw edema and also reduced the level of Evans Blue for vascular permeability. Furthermore, KJD effectively reduced the positive responses for C-reactive protein and rheumatoid arthritis test in FCA-treated rats. Of note, KJD inhibited the level of lipid peroxide malondialdehyde and enhanced the level of superoxide dismutase in the hepatic tissues of FCA-treated rats. Additionally, KJD abrogated the levels of IL-1β and IL-6 in lipopolysaccharide and IFN-γ-exposed RAW 264.7 cells. Also, KJD reduced the expression of cyclooxygenase 2 or inducible nitric oxide synthase at protein and mRNA levels in IFN-γ and lipopolysaccharide-exposed RAW 264.7 cells. Overall, our findings demonstrate that KJD exerts antiinflammatory and analgesic effects via enhancement of antioxidant activity and inhibition of proinflammatory cytokines. Copyright © 2017 John Wiley & Sons, Ltd.
尽管三味药用植物（紫草、阴香和丹参）的草药合剂Kaejadan（KJD）传统上用于治疗类风湿性关节炎，但其科学依据尚未完全明确。因此，我们在体内和体外研究了KJD的抗炎和镇痛机制。Kaejadan抑制了用乙酸处理的小鼠的扭体反应次数，并通过甩尾试验显示出镇痛作用。Kaejadan消除了血清素、角叉菜胶或弗氏完全佐剂（FCA）诱导的爪肿胀，还降低了伊文思蓝的血管通透性水平。此外，KJD有效降低了FCA处理的大鼠中C反应蛋白和类风湿性关节炎试验的阳性反应。值得注意的是，KJD抑制了FCA处理的大鼠肝脏组织中脂质过氧化物丙二醛的水平，并提高了超氧化物歧化酶的水平。此外，KJD消除了脂多糖和IFN-γ刺激的RAW 264.7细胞中IL-1β和IL-6的水平。此外，KJD在IFN-γ和脂多糖刺激的RAW 264.7细胞中，在蛋白质和mRNA水平上降低了环氧合酶2或诱导型一氧化氮合酶的表达。总体而言，我们的研究结果表明，KJD通过增强抗氧化活性和抑制促炎细胞因子发挥抗炎和镇痛作用。版权所有©2017约翰威立父子有限公司。
