Department of Emergency Medicine, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, 310003 Zhejiang, China.
Department of Geriatric Medicine, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, 310003 Zhejiang, China.
Biomed Res Int. 2020 Mar 9;2020:5417201. doi: 10.1155/2020/5417201. eCollection 2020.
The primary target is to reveal whether the resuscitation with hypertonic saline (HTS) or hydroxyethyl starch (HES) would have different effects on the myeloid-derived suppressor cell (MDSC) count and monocytic MDSC (M-MDSC)/granulocytic/neutrophilic MDSC (G-MDSC) rate in the peripheral blood, spleen, and bone marrow nucleated cells (BMNC) in a controlled hemorrhagic shock mouse model under secondary bacterial infection attack, comparing to resuscitation with normal saline (NS) in 72 hours.
After hemorrhagic shock with bacteremia, which is induced by bacterial infection attack, comparing to resuscitation with normal saline (NS) in 72 hours. . After hemorrhagic shock with bacteremia, which is induced by 35218 injection, the mice were distributed into control, NS, HTS, and HES groups. The peripheral blood nucleated cells (PBNC), spleen single-cell suspension, and bone marrow nucleated cells were collected. The flow cytometry was used to detect the MDSC, M-MDSC, and G-MDSC.
In PBNC, after resuscitation with NS, the MDSC was continuously higher, while the rate of M-MDSC/G-MDSC were continuously lower ( < 0.05). In HTS, the MDSC varied, higher at 24 and 72 hours ( < 0.05). In HTS, the MDSC varied, higher at 24 and 72 hours ( < 0.05). In HTS, the MDSC varied, higher at 24 and 72 hours ( < 0.05). In HTS, the MDSC varied, higher at 24 and 72 hours ( < 0.05). In HTS, the MDSC varied, higher at 24 and 72 hours ( < 0.05), the M-MDSC/G-MDSC were continuously lower ( < 0.05). In the spleen, resuscitation with HTS, the M-MDSC/G-MDSC were continuously lower ( < 0.05). In BMNC, after resuscitation with HES, the M-MDSC/G-MDSC were lower at 24 and 72 hours ( < 0.05).
In mouse hemorrhagic shock model with bacterial infection, the resuscitation with NS, HTS, or HES induced difference changes in MDSC and M-MDSC/G-MDSC, which were time-dependent and organ-specific. Resuscitation with crystalloid, like NS or HTS, showed longer effects on the MDSC and M-MDSC/G-MDSC in peripheral blood; while HTS has a longer effect on M-MDSC/G-MDSC in the spleen, HES has a stronger impact on the differentiation regulation of MDSC to G-MDSC in the bone marrow.
本研究旨在探讨在继发性细菌感染攻击导致失血性休克小鼠模型中，与生理盐水（NS）复苏相比，高渗盐水（HTS）或羟乙基淀粉（HES）复苏对周围血、脾脏和骨髓有核细胞（BMNC）中髓系来源抑制细胞（MDSC）计数和单核来源 MDSC（M-MDSC）/粒细胞/中性粒细胞 MDSC（G-MDSC）比率的影响，时间为 72 小时。
通过 35218 注射诱导细菌感染攻击导致失血性休克后，将小鼠分为对照组、NS 组、HTS 组和 HES 组。采集外周血有核细胞（PBNC）、脾单细胞悬液和骨髓有核细胞。采用流式细胞术检测 MDSC、M-MDSC 和 G-MDSC。
在 NS 复苏后，PBNC 中的 MDSC 持续升高，而 M-MDSC/G-MDSC 比率持续降低（<0.05）。HTS 组的 MDSC 有所不同，24 小时和 72 小时较高（<0.05）。HTS 组的 MDSC 有所不同，24 小时和 72 小时较高（<0.05）。HTS 组的 MDSC 有所不同，24 小时和 72 小时较高（<0.05）。HTS 组的 MDSC 有所不同，24 小时和 72 小时较高（<0.05），M-MDSC/G-MDSC 持续降低（<0.05）。在脾脏中，HTS 复苏后 M-MDSC/G-MDSC 持续降低（<0.05）。在 BMNC 中，HES 复苏后 24 小时和 72 小时 M-MDSC/G-MDSC 较低（<0.05）。
在伴有细菌感染的小鼠失血性休克模型中，NS、HTS 或 HES 复苏诱导 MDSC 和 M-MDSC/G-MDSC 发生差异变化，这种变化具有时间依赖性和器官特异性。晶体液复苏，如 NS 或 HTS，在外周血中对 MDSC 和 M-MDSC/G-MDSC 的影响时间更长；而 HTS 对脾脏中 M-MDSC/G-MDSC 的影响时间更长，HES 对骨髓中 MDSC 向 G-MDSC 分化调节的影响更强。
