Ruwart M J, Wilkinson K F, Rush B D, Vidmar T J, Peters K M, Henley K S, Appelman H D, Kim K Y, Schuppan D, Hahn E G
Diabetes and Gastrointestinal Diseases Research and Biostatistics, Upjohn Company, Kalamazoo, Michigan 49001.
Hepatology. 1989 Nov;10(5):801-6. doi: 10.1002/hep.1840100509.
To determine whether a serum parameter of collagen metabolism, serum procollagen type III peptide, correlated with hepatic collagen in a model of diet-induced fibrosis, rats were fed a control or cirrhogenic diet for 6 months and treated with either subcutaneous vehicle or the hepatoprotective prostaglandin 16,16-dimethyl prostaglandin E2 (100 micrograms per kg) twice daily. Pair-fed rats from each group were killed after 2, 4 or 6 months. The value of serum procollagen type III peptide to body weight integrated over time (Kt) correlated linearly with hepatic hydroxyproline content (r = 0.97) at killing time t. Good correlations were also seen between Kt and histopathological assessment of aniline blue-stainable collagen (r = 0.93) and between the histopathology and hydroxyproline content (r = 0.97). Rats receiving 16,16-dimethyl prostaglandin E2 had lower values of all three parameters compared to rats receiving vehicle, confirming the previously demonstrated hepatoprotective effect of 16,16-dimethyl prostaglandin E2. The excellent correlation between Kt and the two other traditional parameters of hepatic collagen suggest that sequential measurements of serum procollagen type III peptide can be used to predict alterations in liver collagen deposition in rats.
为了确定在饮食诱导的肝纤维化模型中，胶原蛋白代谢的血清参数——血清III型前胶原肽是否与肝脏胶原蛋白相关，将大鼠分为两组，分别给予对照饮食或致肝硬化饮食6个月，并分别皮下注射赋形剂或肝保护前列腺素16,16-二甲基前列腺素E2（100微克/千克），每日两次。每组配对饲养的大鼠在2、4或6个月后处死。在处死时间t，血清III型前胶原肽与体重随时间积分值（Kt）与肝脏羟脯氨酸含量呈线性相关（r = 0.97）。Kt与苯胺蓝可染胶原蛋白的组织病理学评估之间（r = 0.93）以及组织病理学与羟脯氨酸含量之间（r = 0.97）也存在良好的相关性。与接受赋形剂的大鼠相比，接受16,16-二甲基前列腺素E2的大鼠这三个参数的值均较低，证实了先前证明的16,16-二甲基前列腺素E2的肝保护作用。Kt与肝脏胶原蛋白的其他两个传统参数之间的良好相关性表明，连续测量血清III型前胶原肽可用于预测大鼠肝脏胶原蛋白沉积的变化。
