Sammra Osama, Rau Jörg, Wickhorst Jörn-Peter, Alssahen Mazen, Hassan Abdulwahed Ahmed, Lämmler Christoph, Kämpfer Peter, Glaeser Stefanie P, Busse Hans-Jürgen, Kleinhagauer Tanita, Knauf-Witzens Tobias, Prenger-Berninghoff Ellen, Abdulmawjood Amir, Klein Günter
Institut für Pharmakologie und Toxikologie, Justus-Liebig-Universität Gießen, 35392 Gießen, Germany.
Chemisches und Veterinärunterschungsamt Stuttgart (CVUAS), Fellbach, Germany.
Int J Syst Evol Microbiol. 2017 Jul;67(7):2093-2097. doi: 10.1099/ijsem.0.001784. Epub 2017 Jul 5.
A taxonomic study using a polyphasic approach was performed on an unidentified Arcanobacterium-like Gram-stain-positive bacterium isolated from the genital tract of a rhinoceros. Comparative 16S rRNA gene sequencing showed that the bacterium belonged to the genus Arcanobacterium and was most closely related to the type strains of Arcanobacterium canis (98.8 % 16S rRNA gene sequence similarity), Arcanobacterium phocisimile (97.8 %), Arcanobacterium phocae (97.7 %), Arcanobacterium haemolyticum (97.4 %), Arcanobacterium hippocoleae (96.6 %), Arcanobacterium pinnipediorum (96.4 %) and Arcarnobacterium pluranimalium (95.4 %). DNA-DNA hybridization values between strain 647T and Arcanobacterium canisDSM 25104T were very low, 13.4 % (reciprocal 15.9 %). The genomic DNA G+C content of strain 647T was 58.7 mol%. The presence of the major menaquinone MK-9(H4) supported the affiliation of this strain to the genus Arcanobacterium. The polar lipid profile consisted of the major components diphosphatidylglycerol, phosphatidylcholine and an unidentified phosphoglycolipid. The results of physiological and biochemical testing clearly distinguished the unknown bacterium from other species of the genus Arcanobacterium. Based on these tests, it is proposed that the unknown bacterium should be classified as a representative of a novel species of the genus Arcanobacterium named Arcanobacterium wilhelmaesp. nov. The type strain is 647T (=DSM 102162T=LMG 29418T).
采用多相分类法对从犀牛生殖道分离出的一株未鉴定的类隐秘杆菌属革兰氏染色阳性细菌进行了分类学研究。比较16S rRNA基因测序表明，该细菌属于隐秘杆菌属，与犬隐秘杆菌模式菌株关系最为密切（16S rRNA基因序列相似性为98.8%），与拟海豹隐秘杆菌（97.8%）、海狗隐秘杆菌（97.7%）、溶血隐秘杆菌（97.4%）、海马隐秘杆菌（96.6%）、鳍足类隐秘杆菌（96.4%）和多动物隐秘杆菌（95.4%）关系较近。菌株647T与犬隐秘杆菌DSM 25104T之间的DNA-DNA杂交值非常低，为13.4%（反向为15.9%）。菌株647T的基因组DNA G+C含量为58.7 mol%。主要甲基萘醌MK-9（H4）的存在支持该菌株属于隐秘杆菌属。极性脂质谱的主要成分包括双磷脂酰甘油、磷脂酰胆碱和一种未鉴定的磷酸糖脂。生理生化测试结果清楚地将该未知细菌与隐秘杆菌属的其他物种区分开来。基于这些测试，建议将该未知细菌归类为隐秘杆菌属一个新物种的代表，命名为威廉姆斯隐秘杆菌（Arcanobacterium wilhelmae）。模式菌株为647T（=DSM 102162T=LMG 29418T）。
