Sujarit Kanaporn, Sujada Nikhom, Kudo Takuji, Ohkuma Moriya, Pathom-Aree Wasu, Lumyong Saisamorn
Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.
Japan Collection of Microorganisms, RIKEN BioResource Center, 3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan.
Int J Syst Evol Microbiol. 2017 Aug;67(8):2773-2778. doi: 10.1099/ijsem.0.002017.
A novel Gram-stain-positive bacterium designated CMU-NKS-70T was isolated from a subterranean termite nest and characterized using a polyphasic approach. The strain exhibited branching, pinkish-cream aerial mycelium and cream-brown substrate mycelium, and formed chains of rod-like spores. The 16S rRNA gene sequence analyses indicated that strain CMU-NKS-70T belonged to the genus Pseudonocardia, showing high similarity with Pseudonocardia oroxyli D10T (98.9 % 16S rRNA gene sequence similarity), Pseudonocardia xishanensis YIM 63638T (98.9 %) and Pseudonocardia kujensis A4038T (98.5 %). However, DNA-DNA relatedness values between strains CMU-NKS-70T and the closest phylogenetically related species ranged from 40.5±2.9 to 48.6±0.7 %. Whole-cell hydrolysates of strain CMU-NKS-70T consisted of meso-diaminopimelic acid, glucose, galactose, arabinose, mannose, ribose and rhamnose. The predominant menaquinone was MK-8(H4). The major cellular fatty acids (>10 %) were iso-C16 : 0, C16 : 0, C16 : 1ω7c and/or iso-C15 : 0 2-OH and 10-methyl C16 : 0. The polar lipids detected were phosphatidylethanolamine, phosphatidylmethylethanolamine, hydroxyphosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, three unidentified glycolipids and two unidentified phospholipids. The G+C content of genomic DNA was 71.9 mol%. The physiological and biochemical properties also supported the phenotypic distinction of strain CMU-NKS-70T from its closely related species. On the basis of evidence from this study using a polyphasic approach, strain CMU-NKS-70T represents a novel species of the genus Pseudonocardia for which the name Pseudonocardia thailandensis sp. nov. is proposed. The type strain is CMU-NKS-70T (=JCM 31292T=TBRC 2000T).
从一个地下白蚁巢中分离出一株新的革兰氏阳性菌，命名为CMU-NKS-70T，并采用多相分类法对其进行了表征。该菌株呈现出分支状、浅粉色至奶油色的气生菌丝体和奶油棕色的基内菌丝体，并形成杆状孢子链。16S rRNA基因序列分析表明，CMU-NKS-70T菌株属于假诺卡氏菌属，与奥氏假诺卡氏菌D10T（16S rRNA基因序列相似性为98.9%）、西山假诺卡氏菌YIM 63638T（98.9%）和库氏假诺卡氏菌A4038T（98.5%）具有高度相似性。然而，CMU-NKS-70T菌株与系统发育关系最密切的物种之间的DNA-DNA杂交值在40.5±2.9%至48.6±0.7%之间。CMU-NKS-70T菌株的全细胞水解产物由内消旋二氨基庚二酸、葡萄糖、半乳糖、阿拉伯糖、甘露糖、核糖和鼠李糖组成。主要的甲基萘醌是MK-8(H4)。主要的细胞脂肪酸（>10%）为异-C16 : 0、C16 : 0、C16 : 1ω7c和/或异-C15 : 0 2-OH以及10-甲基-C16 : 0。检测到的极性脂质有磷脂酰乙醇胺、磷脂酰甲基乙醇胺、羟基磷脂酰乙醇胺、磷脂酰甘油、磷脂酰肌醇、三种未鉴定的糖脂和两种未鉴定的磷脂。基因组DNA的G+C含量为71.9 mol%。生理生化特性也支持CMU-NKS-70T菌株与其密切相关物种在表型上的差异。基于本研究采用多相分类法获得的证据，CMU-NKS-70T菌株代表假诺卡氏菌属的一个新物种，为此提出泰国假诺卡氏菌新种的名称。模式菌株为CMU-NKS-70T（=JCM 31292T=TBRC 2000T）。
