Sammra Osama, Foster Geoffrey, Hassan Abdulwahed Ahmed, Alssahen Mazen, Lämmler Christoph, Glaeser Stefanie P, Kämpfer Peter, Busse Hans-Jürgen, Borowiak Maria, Malorny Burkhard, Ritchie Catriona M, Prenger-Berninghoff Ellen, Abdulmawjood Amir
Institut für Hygiene und Infektionskrankheiten der Tiere, Justus-Liebig-Universität Gießen, 35392 Gießen, Germany.
SRUC Veterinary Services, An Lochran,10 Inverness Campus,Inverness IV2 5NA, UK.
Int J Syst Evol Microbiol. 2020 Jul;70(7):4105-4110. doi: 10.1099/ijsem.0.004230.
A polyphasic taxonomic study was performed on an unidentified -like Gram-stain-positive bacterium designated strain C605018/01/1 isolated from a milk sample collected from the udder of a cow at . Comparative 16S rRNA gene sequencing showed that the bacterium belonged to the genus and was most closely related to the type strain of (99.76 %); sequence similarities to all other species were below 97 %. The wet-lab DNA-DNA hybridization values among strain C605018/01/1 and DSM 13483ᵀ were low, 16.9 % (reciprocal, 49.8 %). Pertaining to the whole genome sequence with a total length of 2.02 Mb and 1654 protein counts, the novel strain C605018/01/01 displayed a G+C content of 51.6 % mol%. The presence of the major menaquinone MK-9(H) supported the affiliation of this strain to the genus . The polar lipid profile consisted of the major components diphosphatidylglycerol, phosphatidylcholine, phosphatidylinositol, phosphatidylinositol-mannoside and unidentified glycolipid and aminophospholipids. Based on these results it is proposed that strain C605018/01/1 should be classified as representing a novel species, sp. nov. The type strain C605018/01/1 (CCUG 45425=DSM 107286=BCCM/LMG 30783).
对从一头奶牛乳房采集的牛奶样本中分离出的一株未鉴定的类革兰氏染色阳性细菌（命名为菌株C605018/01/1）进行了多相分类研究。比较16S rRNA基因测序表明，该细菌属于[具体属名未给出]属，与[具体种名未给出]的模式菌株关系最为密切（99.76%）；与所有其他[该属名]物种的序列相似性均低于97%。菌株C605018/01/1与[具体参照菌株名] DSM 13483ᵀ之间的湿实验室DNA-DNA杂交值较低，为16.9%（双向，49.8%）。关于全长2.02 Mb且有1654个蛋白质编码的全基因组序列，新菌株C605018/01/01的G+C含量为51.6 mol%。主要甲基萘醌MK-9(H)的存在支持了该菌株属于[该属名]属。极性脂质谱由主要成分双磷脂酰甘油、磷脂酰胆碱、磷脂酰肌醇、磷脂酰肌醇甘露糖苷以及未鉴定的糖脂和氨基磷脂组成。基于这些结果，建议将菌株C605018/01/1分类为一个新物种，[新物种名] sp. nov.。模式菌株为C605018/01/1（CCUG 45425 = DSM 107286 = BCCM/LMG 30783）。
