Institute for Infectious Diseases of Animals, Justus Liebig University Gießen, Frankfurter Straße 85-91, D-35392 Gießen, Germany.
Institute of Food Quality and Food Safety, University of Veterinary Medicine Hannover, Bischofsholer Damm 15, 30173 Hannover, Germany.
Int J Syst Evol Microbiol. 2022 Oct;72(10). doi: 10.1099/ijsem.0.005573.
A polyphasic taxonomic study was performed on an unidentified previously described -like Gram-positive strain 2701 isolated from an anal swab of a dead male harbour seal. Comparative 16S rRNA sequencing showed that the bacterium belonged to the genus in the family . The genome sequence of the strain was obtained by Borowiak . [1]. The genome had a G+C content of 49 mol% and a total length of 1.94 Mb. The presence of the major menaquinone MK-9(H) supported the affiliation of the isolate with the genus . The polar lipid profile consisted of diphosphatidylglycerol and an unidentified phospholipid as major components and two unidentified lipids, a further unidentified phospholipid, two unidentified phosphoglycolipids as well as phosphatidylglycerol. The major fatty acids were C, C and C. Biochemical and phylogenetic analyses clearly distinguished the isolate from other members of the genus and closely related other species. Based on these results, it is proposed that the unknown sp. strain 2701 should be classified as representing a novel species with the name sp. nov. The type strain is 2701 (=DSM 112952=LMG 32446).
对一株先前描述过的、从一只死亡雄性港海豹肛门拭子中分离到的不明革兰氏阳性菌 2701 菌株进行了多相分类学研究。比较 16S rRNA 测序结果表明，该细菌属于 科 属。该菌株的基因组序列由 Borowiak 获得。[1]。该基因组的 G+C 含量为 49 mol%，总长度为 1.94 Mb。主要的menaquinone MK-9(H)的存在支持了该分离株与属的关联。极性脂谱由二磷脂酰甘油和一种未鉴定的磷脂作为主要成分以及两种未鉴定的脂质、另一种未鉴定的磷脂、两种未鉴定的磷酸甘油酯以及磷脂酰甘油组成。主要脂肪酸为 C16:0、C18:1ω7c 和 C18:0。生化和系统发育分析清楚地区分了该分离株与属内的其他成员以及密切相关的其他种。基于这些结果，建议将未知的 种 2701 菌株分类为代表一个新种，命名为 新种。模式菌株为 2701（=DSM 112952=LMG 32446）。
