Inami Keiko, Mine Yusuke, Tatsuzaki Jin, Mori Chihiro, Mochizuki Masataka
Faculty of Pharmaceutical Sciences, Tokyo University of Science, Yamazaki 2641, Noda, Chiba 278-8510 Japan.
Tokiwa Phytochemical Co. Ltd, Sakura-shi, Japan.
Genes Environ. 2017 Jan 6;39:5. doi: 10.1186/s41021-016-0068-2. eCollection 2017.
A powdered ethanolic extract of root exhibits antimutagenic activity against -methyl--nitrosourea (MNU) based on the Ames assay with TA1535. The aim of this study was to identify the antimutagenic components of the powdered ethanolic extract of root.
The powdered ethanolic extract of root was sequentially suspended in -hexane, carbon tetrachloride, dichloromethane, ethyl acetate, and ethanol, and each solvent soluble fraction and the residue were assayed for antimutagenic activity against MNU in TA1535. The dichloromethane soluble fraction exhibited the highest antimutagenicity and was fractionated several times by silica gel chromatography. The fraction with the highest antimutagenic activity was further purified using HPLC, and the fractions were assayed for antimutagenicity against MNU in TA1535. Finally, five components with antimutagenic activity against MNU were identified as glyurallin A, glyasperin B, licoricidin, 1-methoxyphaseollin, and licoisoflavone B.
The five components were demonstrated to possess an antigenotoxic effect against carcinogenic MNU for the first time. It is important to prevent DNA damage by -nitrosamines for cancer chemoprevention.
基于用TA1535进行的艾姆斯试验，某植物根的乙醇粉末提取物对N-甲基-N-亚硝基脲（MNU）表现出抗诱变活性。本研究的目的是鉴定该植物根乙醇粉末提取物中的抗诱变成分。
将该植物根的乙醇粉末提取物依次悬浮于正己烷、四氯化碳、二氯甲烷、乙酸乙酯和乙醇中，并检测每种溶剂可溶部分及残留物对TA1535中MNU的抗诱变活性。二氯甲烷可溶部分表现出最高的抗诱变性，并通过硅胶柱色谱法进行了多次分离。具有最高抗诱变活性的部分通过高效液相色谱进一步纯化，并检测这些部分对TA1535中MNU的抗诱变性。最后，鉴定出对MNU具有抗诱变活性的五种成分，分别为glyurallin A、glyasperin B、licoricidin、1-甲氧基菜豆素和licoisoflavone B。
首次证明这五种成分对致癌性MNU具有抗基因毒性作用。通过亚硝胺预防DNA损伤对于癌症化学预防很重要。
