Crandall Leah, Zaman Rashaduz, Ishangulyyeva Guncha, Erbilgin Nadir
Department of Renewable Resources, University of Alberta, Edmonton, AB T6G 2H1, Canada.
Metabolites. 2025 Jul 20;15(7):488. doi: 10.3390/metabo15070488.
BACKGROUND/OBJECTIVES: Within their host trees, mountain pine beetles (MPBs, ) interact with many fungal species, each releasing a unique profile of volatile organic compounds (VOCs). The FVOCs released by the two primary symbionts of MPBs, and , have been found to enhance MPB attraction in the field and laboratory studies. Opportunistic, saprophytic fungal species, such as sp. and , are also common in MPB galleries and can negatively impact MPB fitness. However, little is known about the FVOCs produced by these fungal species and how they may impact MPB feeding and attraction.
To address this knowledge gap, we characterized the FVOC profile of , and performed bioassays to test the effects of its FVOCs on MPB attraction and feeding activity.
Our chemical analysis revealed several FVOCs from known to inhibit the growth of competing fungal species and impact subcortical-beetle attraction.
From those FVOCs, we recommended four compounds-2-pentanone, 2-heptanone, 2-pentanol, and phenylethyl alcohol-for use in future field tests as anti-attraction lures for MPBs. In bioassays, we also observed strong MPB repellency from FVOCs released by , as well as the mild effects of FVOCs on MPB feeding activity. Our findings highlight the potential for these FVOCs to be utilized in the development of more effective MPB anti-attractant lures, which are crucial for the monitoring and management of low-density MPB populations.
背景/目的：在其寄主树木内，山松甲虫（MPB）与许多真菌物种相互作用，每种真菌都会释放出独特的挥发性有机化合物（VOC）谱。在野外和实验室研究中发现，MPB的两种主要共生菌释放的真菌挥发性有机化合物（FVOC）可增强对MPB的吸引力。机会性腐生真菌物种，如某菌种和某菌种，在MPB虫道中也很常见，并且可能对MPB的适合度产生负面影响。然而，对于这些真菌物种产生的FVOC及其如何影响MPB取食和吸引力知之甚少。
为填补这一知识空白，我们对某菌种的FVOC谱进行了表征，并进行了生物测定，以测试其FVOC对MPB吸引力和取食活动的影响。
我们的化学分析揭示了来自某菌种的几种FVOC，已知它们可抑制竞争真菌物种的生长并影响皮层下甲虫的吸引力。
从这些FVOC中，我们推荐了四种化合物——2-戊酮、2-庚酮、2-戊醇和苯乙醇——用于未来的野外试验，作为MPB的抗吸引诱饵。在生物测定中，我们还观察到某菌种释放的FVOC对MPB有很强的驱避作用，以及FVOC对MPB取食活动的轻微影响。我们的研究结果突出了这些FVOC在开发更有效的MPB抗吸引诱饵方面的潜力，这对于低密度MPB种群的监测和管理至关重要。
