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牙龈拟杆菌甘氨酰脯氨酰蛋白酶的表面定位

Surface location of a Bacteroides gingivalis glycylprolyl protease.

作者信息

Grenier D, McBride B C

机构信息

Department of Microbiology, University of British Columbia, Vancouver, Canada.

出版信息

Infect Immun. 1989 Nov;57(11):3265-9. doi: 10.1128/iai.57.11.3265-3269.1989.

DOI:10.1128/iai.57.11.3265-3269.1989
PMID:2807524
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC259793/
Abstract

Various immunological methods were used to localize a glycylprolyl protease previously isolated from Bacteroides gingivalis ATCC 33277. The results obtained by enzyme-linked immunosorbent assay, indirect immunofluorescence staining, and indirect immunogold labeling suggest that the glycylprolyl protease is present on the surface of the cell outer membrane and is specific to B. gingivalis strains. The enzyme was removed from the cell envelope by treatment of the whole cells with sodium dodecyl sulfate, Triton X-100, sodium deoxycholate, and proteinase K.

摘要

采用多种免疫学方法对先前从牙龈卟啉单胞菌ATCC 33277中分离出的甘氨酰脯氨酰蛋白酶进行定位。通过酶联免疫吸附测定、间接免疫荧光染色和间接免疫金标记获得的结果表明，甘氨酰脯氨酰蛋白酶存在于细胞外膜表面，且对牙龈卟啉单胞菌菌株具有特异性。用十二烷基硫酸钠、吐温X-100、脱氧胆酸钠和蛋白酶K处理完整细胞后，该酶从细胞包膜中去除。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aab1/259793/1caa438c0a0b/iai00071-0020-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aab1/259793/4cfba73aada1/iai00071-0018-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aab1/259793/6578a17de773/iai00071-0019-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aab1/259793/93724f5c2857/iai00071-0019-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aab1/259793/1caa438c0a0b/iai00071-0020-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aab1/259793/4cfba73aada1/iai00071-0018-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aab1/259793/6578a17de773/iai00071-0019-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aab1/259793/93724f5c2857/iai00071-0019-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aab1/259793/1caa438c0a0b/iai00071-0020-a.jpg

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本文引用的文献

1
Degradation of collagenous substrates by Bacteroides melaninogenicus.产黑素拟杆菌对胶原底物的降解作用。
J Bacteriol. 1961 Apr;81(4):614-21. doi: 10.1128/jb.81.4.614-621.1961.
2
Degradation of immunoglobulins A2, A2, and G by suspected principal periodontal pathogens.疑似主要牙周病原体对免疫球蛋白A2、A2和G的降解作用。
Infect Immun. 1981 Dec;34(3):757-65. doi: 10.1128/iai.34.3.757-765.1981.
3
Degradation of albumin, haemopexin, haptoglobin and transferrin, by black-pigmented Bacteroides species.
Characterization of recombinant and native forms of a cell surface antigen of Porphyromonas (Bacteroides) gingivalis.
牙龈卟啉单胞菌（拟杆菌属）细胞表面抗原重组形式和天然形式的特性分析
Infect Immun. 1993 Aug;61(8):3294-303. doi: 10.1128/iai.61.8.3294-3303.1993.
4
Depolarization of polymorphonuclear leukocytes by Porphyromonas (Bacteroides) gingivalis 381 in the absence of respiratory burst activation.牙龈卟啉单胞菌（拟杆菌属）381在无呼吸爆发激活情况下对多形核白细胞的去极化作用
Infect Immun. 1991 Sep;59(9):3134-42. doi: 10.1128/iai.59.9.3134-3142.1991.
J Med Microbiol. 1984 Aug;18(1):39-46. doi: 10.1099/00222615-18-1-39.
4
Black-pigmented Bacteroides species, Capnocytophaga species, and Actinobacillus actinomycetemcomitans in human periodontal disease: virulence factors in colonization, survival, and tissue destruction.人类牙周病中的黑色普氏菌属、二氧化碳嗜纤维菌属和伴放线放线杆菌：定植、存活及组织破坏中的毒力因子
J Dent Res. 1984 Mar;63(3):412-21. doi: 10.1177/00220345840630031101.
5
Procedure for isolation of bacterial lipopolysaccharides from both smooth and rough Pseudomonas aeruginosa and Salmonella typhimurium strains.从光滑型和粗糙型铜绿假单胞菌及鼠伤寒沙门氏菌菌株中分离细菌脂多糖的方法。
J Bacteriol. 1983 Aug;155(2):831-8. doi: 10.1128/jb.155.2.831-838.1983.
6
"Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate--polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A.“蛋白质免疫印迹法”：蛋白质从十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳转移至未修饰的硝酸纤维素膜上，并用抗体和放射性碘化蛋白A进行放射自显影检测。
Anal Biochem. 1981 Apr;112(2):195-203. doi: 10.1016/0003-2697(81)90281-5.
7
Degradation of the human proteinase inhibitors alpha-1-antitrypsin and alpha-2-macroglobulin by Bacteroides gingivalis.牙龈卟啉单胞菌对人类蛋白酶抑制剂α-1-抗胰蛋白酶和α-2-巨球蛋白的降解作用
Infect Immun. 1984 Feb;43(2):644-8. doi: 10.1128/iai.43.2.644-648.1984.
8
Association of oral Bacteroides with gingivitis and adult periodontitis.口腔拟杆菌与牙龈炎和成人牙周炎的关联。
J Periodontal Res. 1981 May;16(3):259-65. doi: 10.1111/j.1600-0765.1981.tb00974.x.
9
Black-pigmented Bacteroides spp. in the human oral cavity.人类口腔中的产黑色素拟杆菌属
Infect Immun. 1981 Apr;32(1):198-203. doi: 10.1128/iai.32.1.198-203.1981.
10
Characterization of a trypsin-like protease from the bacterium Bacteroides gingivalis isolated from human dental plaque.从人类牙菌斑中分离出的牙龈拟杆菌中一种类胰蛋白酶的特性研究
Arch Oral Biol. 1984;29(7):559-64. doi: 10.1016/0003-9969(84)90078-5.