Helmberg A, Böck G, Wolf H, Wick G
Institute for General and Experimental Pathology, University of Innsbruck Medical School, Austria.
Infect Immun. 1989 Nov;57(11):3576-80. doi: 10.1128/iai.57.11.3576-3580.1989.
To assess the potential effect of an orally administered immunomodulator, consisting of a lysate of seven different bacteria, on polymorphonuclear leukocyte (PMN) function, rabbits were fed this preparation for five consecutive days via a gastric tube. On day 6, PMN were separated from peripheral blood and oxidative burst was triggered by opsonized zymosan or 12-O-tetradecanoylphorbol-13-acetate and quantitated on a single-cell basis. This study presents the extension of an existing flow cytometric method, leading to the possibility of quantitating single-cell oxidative burst triggered by particulate (instead of only soluble) stimuli. By this means, treated animals showed statistically significant increased oxidative burst reactions compared with the control group. The data provide evidence that oral application of a bacterial immunomodulator leads to a primed state in PMN for increased oxidative activity in response to a particulate stimulus. This offers the possibility that the beneficial effect of similar treatment in humans may in part be due to comparable mechanisms.
为评估一种由七种不同细菌的裂解物组成的口服免疫调节剂对多形核白细胞（PMN）功能的潜在影响，通过胃管连续五天给兔子喂食该制剂。在第6天，从外周血中分离出PMN，并用调理酵母聚糖或12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯触发氧化爆发，并在单细胞基础上进行定量。本研究展示了对现有流式细胞术方法的扩展，使得定量由颗粒性（而非仅可溶性）刺激引发的单细胞氧化爆发成为可能。通过这种方法，与对照组相比，经治疗的动物显示出氧化爆发反应有统计学意义的增加。数据提供了证据，表明口服细菌免疫调节剂会使PMN处于预激活状态，以增加对颗粒性刺激的氧化活性。这表明类似治疗对人类的有益作用可能部分归因于类似的机制。
