Goyal S, Aggarwal J, Dubey P K, Mishra B P, Ghalsasi P, Nimbkar C, Joshi B K, Kataria R S
a Animal Biotechnology Division , National Bureau of Animal Genetic Resources , Karnal , Haryana , India.
b RIKEN Center for Life Science Technologies , Yokohama , Japan.
Anim Biotechnol. 2017 Jul 3;28(3):220-227. doi: 10.1080/10495398.2016.1262869. Epub 2017 Jan 11.
The effect of FecB mutation on the gene expression in FecB carrier and noncarrier estrous synchronized ewes, has been analyzed. For this study the whole ovarian tissues and Graafian follicles were collected from estrus synchronized FecB carrier Garole, and non-carrier Deccani Indian sheep, showing remarkable differences in the numbers of preovulatory follicles among two groups. Eleven potential candidate genes (BMP15, GDF9, BMP4, BMP7, BMPR1B, BMPR1A, SMAD9, LHCGR, FSHR, IGF1R, and STAT5) were selected for their expression analysis by SybrGreen based real-time PCR, across ovaries and Graafian follicles of different fecundity groups, for having better insights into the effect of FecB genotypes on follicular development. Variable expression was observed for almost all the genes included in the present study among high and low fecundity groups that was most significant for the BMP7, BMP4, LHCGR, and FSHR transcripts in the ovarian follicles of high and low fecundity ewes, indicating their importance in governing the fecundity in FecB carrier, Indian Garole sheep. BMP4 expression among the genes studied was significantly higher in FecB carrier Garole sheep. This study confirms the changes in mRNA expression of the genes implicated in follicular development in FecB carrier and noncarrier Indian sheep breeds.
分析了FecB突变对FecB基因携带者和非携带者发情同步母羊基因表达的影响。在本研究中，从发情同步的FecB基因携带者加罗尔羊和非携带者德卡尼印度绵羊中采集了整个卵巢组织和格拉夫卵泡，两组在排卵前卵泡数量上存在显著差异。选择了11个潜在的候选基因（BMP15、GDF9、BMP4、BMP7、BMPR1B、BMPR1A、SMAD9、LHCGR、FSHR、IGF1R和STAT5），通过基于SybrGreen的实时PCR对不同繁殖力组的卵巢和格拉夫卵泡进行表达分析，以便更好地了解FecB基因型对卵泡发育的影响。在本研究中纳入的几乎所有基因中，高繁殖力组和低繁殖力组之间均观察到可变表达，其中BMP7、BMP4、LHCGR和FSHR转录本在高繁殖力和低繁殖力母羊的卵巢卵泡中差异最为显著，表明它们在控制FecB基因携带者印度加罗尔羊的繁殖力方面具有重要作用。在所研究的基因中，BMP4在FecB基因携带者加罗尔羊中的表达显著更高。本研究证实了FecB基因携带者和非携带者印度绵羊品种中与卵泡发育相关的基因mRNA表达的变化。
